Document Detail


Adenosine deamination increases the survival under acidic conditions in Escherichia coli.
MedLine Citation:
PMID:  22277147     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims:  Resistance to acidic stress contributes to bacterial persistence in the host and is thought to promote their passage through the human gastric barrier. The aim of this study was to examine whether or not nucleosides have a role in the survival under acidic conditions in Escherichia coli. Methods and Results:  We found that adenosine has a function to survive against extremely acidic stress. The deletion of add encoding adenosine deaminase that converts adenosine into inosine and NH(3) attenuated the survival in the presence of adenosine. The addition of adenosine increased intracellular pH of E. coli cells in pH 2.5 medium. The addition of inosine or adenine did not increase the resistance to acidic conditions. Conclusions:  Our present results imply that adenosine was used to survive under extremely acidic conditions via the production of NH(3) . Significance and Impact of the Study:  It has been proposed that amino acid decarboxylation is the major system for the resistance of E. coli to acidic stress. In the present study, the adenosine deamination was shown to induce the survival under acidic conditions, demonstrating that bacteria have alternative strategies to survive under acidic conditions besides amino acid decarboxylation. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Authors:
Yirong Sun; Toshihiko Fukamachi; Hiromi Saito; Hiroshi Kobayashi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-25
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Affiliation:
Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan.
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