| Adenosine analogue induced ultrastructural changes in the nucleolus that correlate with inhibition of ribosomal RNA processing. | |
| | |
MedLine Citation:
|
PMID: 6608553 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The aminonucleotide of puromycin (AMS) is known to have a differential effect on ribosomal RNA (rRNA) synthesis and the cell-cycle traverse in normal as compared to neoplastic or virally transformed cells. In this study, AMS and its demethylated derivative, 3'-amino-3'-deoxyadenosine (3'-AmA) have been used to compare their effects on normal (IMR 90) versus transformed (AG 2804) human fibroblasts with regard to preribosomal RNA (pre-rRNA) transcription, the processing of this RNA, and structural changes in the nucleolus. The processing of pre-rRNA in normal and transformed fibroblasts treated with 3'-AmA for 4 hr was markedly depressed. However, this process did not appear to be affected by the AMS treatment of normal cells, while in transformed cells it was maximally inhibited within 4 hr of exposure to this drug. Ultrastructural changes were observed in the nucleoli of normal and transformed cells treated with 3'-AmA, whereas treatment of these cells with AMS produced alterations of nucleolar structure only in the transformed cells. These changes correlated with the onset of inhibition of pre-rRNA processing. Our findings suggest that the impairment of pre-rRNA processing produced by AMS and 3'-AmA in transformed cells and by 3'-AmA in normal cells may be due to structural disorganization of the nucleolus produced by these antimetabolites. |
| | |
Authors:
|
A R Tunkel; G P Studzinski |
Related Documents
:
|
221923 - Detection of a transformation-related antigen in chemically induced sarcomas and other ... 20398293 - Genetic transformation of cotton with a harpin-encoding gene hpaxoo confers an enhanced... 15776463 - The role of rhoa in the regulation of cell morphology and motility. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 32 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 1984 Apr |
Date Detail:
|
Created Date: 1984-05-04 Completed Date: 1984-05-04 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 363-71 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Nucleolus
/
drug effects*,
metabolism,
ultrastructure Cell Transformation, Viral Deoxyadenosines / analogs & derivatives*, pharmacology Fibroblasts / drug effects, metabolism, ultrastructure Humans Lung Nucleic Acid Precursors / metabolism Puromycin / analogs & derivatives* Puromycin Aminonucleoside / pharmacology* RNA Precursors RNA Processing, Post-Transcriptional / drug effects* RNA, Ribosomal / antagonists & inhibitors, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
R01-CA19750/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Deoxyadenosines; 0/Nucleic Acid Precursors; 0/RNA Precursors; 0/RNA, Ribosomal; 2504-55-4/3'-amino-3'-deoxyadenosine; 53-79-2/Puromycin; 58-60-6/Puromycin Aminonucleoside |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Gardnerella vaginalis. Frequency of its isolation from women complaining of leukorrhea
Next Document: The influence of common variables on T cell subset analysis by monoclonal antibodies.