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Adenosine A2a receptor induces GDNF expression by the Stat3 signal in vitro.
MedLine Citation:
PMID:  23032401     Owner:  NLM     Status:  In-Data-Review    
Adenosine A2a receptor (A2aR) is believed to play a role in a number of physiological responses and pathological conditions. Our group has shown previously that A2aR-activated astrocytes occurred following oxygen-glucose deprivation. However, the relationship between A2aR and neurotrophins is poorly understood. Here, we investigate the effect of A2aR on glial cell line-derived neurotrophic factor (GDNF) expression in rat brain astrocyte-2 (RBA-2) cells by quantitative PCR and western blot. We established a stable A2aR-overexpressing cell line and found that A2aR induced GDNF expression both at mRNA and protein levels. A2aR-selective antagonist Sch58261 decreased GDNF expression in a dose-dependent manner with increased activation of Stat3. The Stat3 inhibitor reversed the effect of Sch58261 on GDNF expression. Therefore, these data indicate that A2aR induces GDNF expression by the Stat3 pathway, which provides a new insight into the function of A2aR in cerebral ischemia and neuroprotection.
Rong-Hu Ke; Ji Xiong; Ying Liu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuroreport     Volume:  23     ISSN:  1473-558X     ISO Abbreviation:  Neuroreport     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  England    
Other Details:
Languages:  eng     Pagination:  958-62     Citation Subset:  IM    
aState Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine bDepartment of Neuropathology, Institute of Neurology, Huashan Hospital cDepartment of Pathology, Shanghai Medical College, Fudan University, Shanghai, China.
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