Document Detail


Adenosine A1 receptor-mediated modulation of dopamine D1 receptors in stably cotransfected fibroblast cells.
MedLine Citation:
PMID:  9468534     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The antagonistic interactions between adenosine A1 and dopamine D1 receptors were studied in a mouse Ltk- cell line stably cotransfected with human adenosine A1 receptor and dopamine D1 receptor cDNAs. In membrane preparations, both the adenosine A1 receptor agonist N6-cyclopentyladenosine and the GTP analogue guanyl-5'-yl imidodiphospate induced a decrease in the proportion of dopamine D1 receptors in a high affinity state. In the cotransfected cells, the adenosine A1 agonist induced a concentration-dependent inhibition of dopamine-induced cAMP accumulation. Blockade of adenosine A1 receptor signal transduction with the adenosine A1 receptor antagonist 1,3-dipropyl-8-cyclopentylxanthine or with pertussis toxin pretreatment increased both basal and dopamine-stimulated cAMP levels, indicating the existence of tonic adenosine A1 receptor activation. Pretreatment with pertussis toxin also counteracted the effects of low concentrations of the A1 agonist on D1 receptor-agonist binding. The results suggest that adenosine A1 receptors antagonistically modulate dopamine D1 receptors at the level of receptor binding and the generation of second messengers.
Authors:
S Ferré; M Torvinen; K Antoniou; E Irenius; O Civelli; E Arenas; B B Fredholm; K Fuxe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  273     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-19     Completed Date:  1998-03-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4718-24     Citation Subset:  IM    
Affiliation:
Department of Neuroscience, Karolinska Institute, S-171 77 Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzazepines / metabolism
Binding, Competitive
Blotting, Northern
Cell Line
Cyclic AMP / metabolism
DNA, Complementary
Dopamine / metabolism
Dopamine Antagonists / metabolism
Humans
Mice
Receptors, Dopamine D1 / genetics,  metabolism*
Receptors, Purinergic P1 / antagonists & inhibitors,  metabolism*
Signal Transduction
Transfection
Tritium
Xanthines / pharmacology
Chemical
Reg. No./Substance:
0/Benzazepines; 0/DNA, Complementary; 0/Dopamine Antagonists; 0/Receptors, Dopamine D1; 0/Receptors, Purinergic P1; 0/Xanthines; 10028-17-8/Tritium; 102146-07-6/1,3-dipropyl-8-cyclopentylxanthine; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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