Document Detail

Adenosine 5'-monophosphate increases levels of leukotrienes in breath condensate in asthma.
MedLine Citation:
PMID:  15250231     Owner:  NLM     Status:  MEDLINE    
Hyperresponsiveness (AHR) is a key physiological abnormality in asthma. In clinical and research studies AHR is measured bronchial challenge, with methacholine (MCh), but more recently with adenosine-5'-monophosphate (AMP). In the search for markers of airway inflammation in asthmatic patients, we measured the concentrations of histamine and cysteinyl-leukotrienes (cys-LTs) before and after MCh and AMP challenges in the exhaled breath condensate of 13 patients with mild asthma (FEV1 78.5%pred) and nine healthy non-smokers, using specific enzyme immunoassays. With methacholine challenge we did not find any differences between asthmatics and normal subjects in the pre- and post-challenge concentrations of cys-LTs: 27.2+/-1.4 vs. 29.2+/-1.2 pg/ml and 26.3+/-2.2 vs. 27.5+/-4.2 pg/ml, respectively or histamine: 5.1+/-0.4 vs. 5.1+/-0.6 nM and 4.5+/-0.4 vs. 4.4+/-0.3 nM; P>0.05). In asthmatic patients cys-LT levels were significantly higher after AMP challenge (56.2+/-9.7 vs. 31.7+/-6.9 pg/ml; P<0.05); but there was no difference in healthy subjects (27.2+/-4.6 vs. 30.3+/-4.7 pg/ml). There was no difference in histamine concentrations in asthmatic (5.9+/-1.8 vs. 4.5+/-0.5 nM), or healthy subjects (5.5+/-0.4 vs.5.7+/-0.9 nM) after AMP challenge. In conclusion, our results show that the cys-LTs are increased in exhaled breath condensate after AMP challenge, which may indicate that the AMP acts indirectly by releasing cys-LTs from primed mast cells. The detection of LTs and histamine in exhaled breath condensate may be useful in monitoring asthma.
E Bucchioni; Z Csoma; L Allegra; K F Chung; P J Barnes; S A Kharitonov
Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Respiratory medicine     Volume:  98     ISSN:  0954-6111     ISO Abbreviation:  Respir Med     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-14     Completed Date:  2004-09-09     Revised Date:  2005-06-09    
Medline Journal Info:
Nlm Unique ID:  8908438     Medline TA:  Respir Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  651-5     Citation Subset:  IM    
Department of Thoracic Medicine, National Heart and Lung Institute, Imperial College, Dovehouse Street, London SW3 6LY, UK.
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MeSH Terms
Adenosine Monophosphate / diagnostic use*
Asthma / diagnosis*,  physiopathology
Biological Markers / analysis
Breath Tests / methods
Bronchial Hyperreactivity / diagnosis
Bronchial Provocation Tests / methods
Cysteine / metabolism*
Forced Expiratory Volume
Histamine Release / drug effects
Inflammation Mediators / metabolism
Leukotrienes / metabolism*
Methacholine Chloride / diagnostic use
Vital Capacity
Reg. No./Substance:
0/Biological Markers; 0/Inflammation Mediators; 0/Leukotrienes; 0/cysteinyl-leukotriene; 52-90-4/Cysteine; 61-19-8/Adenosine Monophosphate; 62-51-1/Methacholine Chloride
Comment In:
Respir Med. 2005 Apr;99(4):515; author reply 516   [PMID:  15763461 ]

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