| Adeno-associated virus infection of murine fibroblasts with help provided by mouse adenovirus. | |
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MedLine Citation:
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PMID: 19464040 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adeno-associated virus (AAV-2) replicates to high titers when host cells are coinfected with a helper virus. Here we analyzed the coinfection of AAV-2 and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that AAV-2/MAV-1 coinfected NIH 3T3 cells produced approximately 10-40-fold less AAV-2 DNAse resistant particles than Hela cells. Levels of AAV-2 DNA replication were approximately 30-fold less in 3T3 cells as compared to Hela cells coinfected with human adenovirus (Ad-5). A study of these lower levels of infection in 3T3 cells compared to Hela cells revealed that receptor binding and internalization of AAV-2 in 3T3 and Hela cells was comparable. However, AAV-2 did not enter into the nucleus of mouse cells as efficiently as it does in human cells. Furthermore, viral DNA replication levels of AAV-2 DNA were found to be lower in mouse cells than human cells, indicating limitations in the murine nucleus for viral replication. |
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Authors:
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Vipul Bhrigu; James P Trempe |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-05-21 |
Journal Detail:
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Title: Virology Volume: 390 ISSN: 1096-0341 ISO Abbreviation: Virology Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-07-07 Completed Date: 2009-07-24 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 22-30 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Cancer Biology, University of Toledo, College of Medicine, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviruses, Human
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genetics,
physiology Animals Base Sequence Cell Nucleus / virology DNA Replication DNA, Viral / genetics Deoxyribonuclease I Dependovirus / genetics*, physiology* Hela Cells Helper Viruses / genetics*, physiology* Humans Mastadenovirus / genetics*, physiology* Mice NIH 3T3 Cells Plasmids / genetics Receptors, Virus / physiology Species Specificity Transfection Viral Proteins / genetics, metabolism Virus Replication |
| Grant Support | |
ID/Acronym/Agency:
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AI51471/AI/NIAID NIH HHS; GM64765/GM/NIGMS NIH HHS; R01 AI051471-05/AI/NIAID NIH HHS; R01 GM064765-04/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/Receptors, Virus; 0/Viral Proteins; EC 3.1.21.1/Deoxyribonuclease I |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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