Document Detail


Adeno-associated virus infection of murine fibroblasts with help provided by mouse adenovirus.
MedLine Citation:
PMID:  19464040     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adeno-associated virus (AAV-2) replicates to high titers when host cells are coinfected with a helper virus. Here we analyzed the coinfection of AAV-2 and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that AAV-2/MAV-1 coinfected NIH 3T3 cells produced approximately 10-40-fold less AAV-2 DNAse resistant particles than Hela cells. Levels of AAV-2 DNA replication were approximately 30-fold less in 3T3 cells as compared to Hela cells coinfected with human adenovirus (Ad-5). A study of these lower levels of infection in 3T3 cells compared to Hela cells revealed that receptor binding and internalization of AAV-2 in 3T3 and Hela cells was comparable. However, AAV-2 did not enter into the nucleus of mouse cells as efficiently as it does in human cells. Furthermore, viral DNA replication levels of AAV-2 DNA were found to be lower in mouse cells than human cells, indicating limitations in the murine nucleus for viral replication.
Authors:
Vipul Bhrigu; James P Trempe
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-05-21
Journal Detail:
Title:  Virology     Volume:  390     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-07     Completed Date:  2009-07-24     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  22-30     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Cancer Biology, University of Toledo, College of Medicine, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenoviruses, Human / genetics,  physiology
Animals
Base Sequence
Cell Nucleus / virology
DNA Replication
DNA, Viral / genetics
Deoxyribonuclease I
Dependovirus / genetics*,  physiology*
Hela Cells
Helper Viruses / genetics*,  physiology*
Humans
Mastadenovirus / genetics*,  physiology*
Mice
NIH 3T3 Cells
Plasmids / genetics
Receptors, Virus / physiology
Species Specificity
Transfection
Viral Proteins / genetics,  metabolism
Virus Replication
Grant Support
ID/Acronym/Agency:
AI51471/AI/NIAID NIH HHS; GM64765/GM/NIGMS NIH HHS; R01 AI051471-05/AI/NIAID NIH HHS; R01 GM064765-04/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Receptors, Virus; 0/Viral Proteins; EC 3.1.21.1/Deoxyribonuclease I
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Pathological manifestations of feline immunodeficiency virus (FIV) infection in wild African lions.
Next Document:  Sepsis Due to Fluoroquinolone-resistant Escherichia coli After Transrectal Ultrasound-guided Prostat...