| Adenine nucleotides and carbohydrates in subpopulations of hepatic nodules with normal and compromised microcirculation. | |
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MedLine Citation:
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PMID: 2470503 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescein-isothiocyanate dextran (FITC-dextran), a dye confined to the vascular space, was infused via the hepatic artery and portal vein into perfused livers from fed rats treated with diethylnitrosamine for 4 to 5 months. Fluorescence due to FITC-dextran was detected with fiberoptic microlight guides placed on surface nodules of about 5 mm in diameter. Nodules were categorized into groups with normal and compromised microcirculation based on their fluorescence following infusion of FITC-dextran. Similar results were obtained when nodules were classified based on reflectance of trypan blue. Despite compromised microcirculation, ATP and ADP levels as well as ATP/ADP ratios were comparable in both groups of nodules; however, AMP was elevated in FITC-dextran-negative nodules (i.e., those with compromised microcirculation). Nodules with compromised microcirculation also contained higher glucose and lactate levels than nodules that were well perfused; however, glycogen was five times lower than in FITC-dextran-positive nodules. Fasting reduced ATP/ADP ratios in poorly perfused nodules in comparison to well-perfused nodules. In perfused livers from fed rats where glycogen was high, however, ATP/ADP ratios and rates of ATP depletion during ischemia were the same in well-perfused and poorly perfused nodules. Products of glycogen breakdown (e.g., glucose and lactate) were elevated in nodules from livers of fed but not fasted rats. The results indicate that alteration of perfusion of hepatic nodules does not change ATP levels nor the capacity of nodules to utilize high energy phosphate during anoxia. Thus, near normal energy status is maintained from glycogen metabolism in poorly perfused nodules via glycolysis. Since basal ATP content and utilization is comparable in well and poorly perfused nodules, compromised energy status is unlikely to explain selection of nodules that regress to near normal hepatocytes. |
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Authors:
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I Anundi; F C Kauffman; J M te Koppele; H Yamanaka; M Whittaker; J A Popp; R G Thurman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer research Volume: 49 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1989 Jun |
Date Detail:
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Created Date: 1989-07-06 Completed Date: 1989-07-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3282-6 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of North Carolina, Chapel Hill 27514. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine Nucleotides
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metabolism* Animals Carbohydrate Metabolism* Dextrans Diethylnitrosamine Fluorescein-5-isothiocyanate* / analogs & derivatives* Fluoresceins Fluorescent Dyes Glucose / metabolism Lactates / metabolism Liver / metabolism Liver Circulation* Liver Glycogen / metabolism Liver Neoplasms, Experimental / blood supply, metabolism* Male Microcirculation / physiopathology Precancerous Conditions / blood supply, chemically induced, metabolism* Rats Rats, Inbred F344 Spectrometry, Fluorescence |
| Grant Support | |
ID/Acronym/Agency:
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CA-20807/CA/NCI NIH HHS; CA-23080/CA/NCI NIH HHS; ES-02759/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adenine Nucleotides; 0/Fluoresceins; 0/Fluorescent Dyes; 0/Lactates; 0/Liver Glycogen; 0/fluorescein isothiocyanate dextran; 3326-32-7/Fluorescein-5-isothiocyanate; 50-99-7/Glucose; 55-18-5/Diethylnitrosamine; 9004-54-0/Dextrans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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