Document Detail


Addressable droplet microarrays for single cell protein analysis.
MedLine Citation:
PMID:  25262574     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Addressable droplet microarrays are potentially attractive as a way to achieve miniaturised, reduced volume, high sensitivity analyses without the need to fabricate microfluidic devices or small volume chambers. We report a practical method for producing oil-encapsulated addressable droplet microarrays which can be used for such analyses. To demonstrate their utility, we undertake a series of single cell analyses, to determine the variation in copy number of p53 proteins in cells of a human cancer cell line.
Authors:
Ali Salehi-Reyhani; Edward Burgin; Oscar Ces; Keith R Willison; David R Klug
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Analyst     Volume:  139     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-09-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  5367-74     Citation Subset:  IM    
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