Document Detail


Additive effects of lactation and food restriction to increase hypothalamic neuropeptide Y mRNA in rats.
MedLine Citation:
PMID:  9071956     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neuropeptide Y (NPY) is the most powerful appetite stimulant known, and rates of synthesis and release in the hypothalamus correlate closely with nutritional status. Pregnancy and lactation provide an excellent model of physiological hyperphagia. In this study the authors measured food intake, plasma glucose, insulin and luteinizing hormone (LH) and hypothalamic NPY mRNA in rats during pregnancy and in early and late lactation. The effect of food restriction (to 80% of control) during lactation was also studied. Pregnancy resulted in a modest increase in daily food intake over non-lactating controls (controls: 15.6 +/- 0.6 g, pregnant: 19.8 +/- 1.1 g, P < 0.01). During lactation food intake increased dramatically to 355% of non-lactating levels by the 12th day. Insulin and glucose levels were unchanged in lactation, except in the food-restricted animals, when insulin levels were reduced to 49.5 +/- 18.4 pmol/l compared with 215 +/- 55 pmol/l (P < 0.01) in lactating, non-restricted animals, and glucose was reduced to 3.7 +/- 0.2 mmol/l compared with 5.1 +/- 0.2 mmol/l in non-restricted lactating animals. Hypothalamic NPY mRNA was unchanged in pregnancy, moderately increased after 5 days lactation (130 +/- 6.2% of control, P < 0.01) and increased further at 14 days lactation (179 +/- 14%, P < 0.001). The greatest changes occurred in the animals who were food-deprived during lactation, when hypothalamic NPY mRNA levels reached 324 +/- 44% (P < 0.001) of non-lactating levels. Increases in hypothalamic NPY synthesis may be partly responsible for the increase in food intake seen in lactation, but unlike in food deprivation, the increase is not related to circulating insulin, suggesting involvement of other regulatory factors.
Authors:
J P Wilding; M O Ajala; P D Lambert; S R Bloom
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  152     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-04-02     Completed Date:  1997-04-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  365-9     Citation Subset:  IM    
Affiliation:
Division of Endocrinology and Metabolism, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Glucose / metabolism
Eating
Female
Food Deprivation*
Hypothalamus / metabolism*
Insulin / blood
Lactation / physiology*
Luteinizing Hormone / blood
Neuropeptide Y / genetics*
Pregnancy
RNA, Messenger / metabolism*
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Neuropeptide Y; 0/RNA, Messenger; 11061-68-0/Insulin; 9002-67-9/Luteinizing Hormone

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