Document Detail


Additive effects of Cox-1 and Cox-2 inhibition on breast cancer in vitro.
MedLine Citation:
PMID:  16964399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We hypothesized that combined treatment with Cox-1 and Cox-2 specific inhibitiors would exhibit synergistic effects against breast cancer in vitro. Two human breast cancer cell lines (HTB26, MCF-7) were treated with catechin (Cox-1 inhibitor) or NS398 (Cox-2 inhibitor) at 100 microM as both single and combined treatments. Reductions in cell growth were observed in both cell lines at 24 and 72 h in both single and combined treatments (p<0.001). Combined treatment produced a significantly greater inhibition as compared to single agents alone. Upon cell cycle evaluation, Cox-1 and -2 antagonism increased G1 and G2 phase fractions in MCF-7 cells (p<0.001 and p<0.05 respectively). No additive changes were observed when the two agents were combined. An increase in the G2 phase was observed in the HTB26 cells when treated with NS398 alone (p<0.001). However, a decrease in the S-phase was observed when these cells were treated with NS398, as a single agent (p<0.01) or when the two agents were combined (p<0.01). The significant and additive effects exhibited by the combination of Cox-1 and -2 inhibitors and their effects on cell cycle suggest that these agents could become an effective treatment modality for carcinoma of the breast.
Authors:
David W McFadden; Dale R Riggs; Barbara J Jackson; Cynthia Cunningham
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of oncology     Volume:  29     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-11     Completed Date:  2006-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1019-23     Citation Subset:  IM    
Affiliation:
Department of Surgery, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV 26506, USA. dmcfadden@hsc.wvu.edu
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / enzymology*
Cell Cycle / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Cyclooxygenase 1 / drug effects*
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors / pharmacology*
Cyclooxygenase Inhibitors / pharmacology*
Drug Synergism
Female
Humans
Membrane Proteins / antagonists & inhibitors*
Chemical
Reg. No./Substance:
0/Cyclooxygenase 2 Inhibitors; 0/Cyclooxygenase Inhibitors; 0/Membrane Proteins; EC 1.14.99.1/Cyclooxygenase 1; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human

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