Document Detail


Additional sex combs-like 1 belongs to the enhancer of trithorax and polycomb group and genetically interacts with Cbx2 in mice.
MedLine Citation:
PMID:  19833123     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Additional sex combs (Asx) gene of Drosophila behaves genetically as an enhancer of trithorax and polycomb (ETP) in displaying bidirectional homeotic phenotypes, suggesting that is required for maintenance of both activation and silencing of Hox genes. There are three murine homologs of Asx called Additional sex combs-like1, 2, and 3. Asxl1 is required for normal adult hematopoiesis; however, its embryonic function is unknown. We used a targeted mouse mutant line Asxl1(tm1Bc) to determine if Asxl1 is required to silence and activate Hox genes in mice during axial patterning. The mutant embryos exhibit simultaneous anterior and posterior transformations of the axial skeleton, consistent with a role for Asxl1 in activation and silencing of Hox genes. Transformations of the axial skeleton are enhanced in compound mutant embryos for the polycomb group gene M33/Cbx2. Hoxa4, Hoxa7, and Hoxc8 are derepressed in Asxl1(tm1Bc) mutants in the antero-posterior axis, but Hoxc8 expression is reduced in the brain of mutants, consistent with Asxl1 being required both for activation and repression of Hox genes. We discuss the genetic and molecular definition of ETPs, and suggest that the function of Asxl1 depends on its cellular context.
Authors:
C L Fisher; I Lee; S Bloyer; S Bozza; J Chevalier; A Dahl; C Bodner; C D Helgason; J L Hess; R K Humphries; H W Brock
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-10-13
Journal Detail:
Title:  Developmental biology     Volume:  337     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-11-27     Completed Date:  2009-12-15     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9-15     Citation Subset:  IM    
Affiliation:
Department of Zoology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T1Z3, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Bone and Bones / abnormalities*
DNA-Binding Proteins / genetics
Female
Mice
Mice, Inbred C57BL
Mutation
Phenotype
Pregnancy
Repressor Proteins / genetics,  physiology*
Spine / abnormalities
Grant Support
ID/Acronym/Agency:
R01 CA078815-03/CA/NCI NIH HHS; R01 CA092251-11/CA/NCI NIH HHS; R01-CA-0078815/CA/NCI NIH HHS; //Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Asxl1 protein, mouse; 0/DNA-Binding Proteins; 0/Hoxa4 protein, mouse; 0/Repressor Proteins; 0/polycomb group proteins
Comments/Corrections

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