| Addition of hypotaurine can reactivate immotile golden hamster spermatozoa. | |
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MedLine Citation:
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PMID: 2312401 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hamster epididymal spermatozoa became virtually immotile following washing and dilution in chemically defined medium (TLP-PVA). The sperm motility factors (penicillamine, hypotaurine, and epinephrine: PHE) were examined for their ability to reactivate immotile sperm. Sperm could be reactivated by addition of PHE at 1 h of incubation. Hypotaurine alone was capable of reactivating sperm motility, but epinephrine and penicillamine together were not. However, overall sperm motility and percentage of motile sperm during incubation were higher when PHE components were used in combination than when hypotaurine was used alone. Addition of hypotaurine to immotile sperm suspensions could be postponed for up to 6 h with subsequent recovery of sperm motility, although the degree of recovery of motility declined progressively with each hour that addition of hypotaurine was delayed. The rescuing effect of hypotaurine was due to an increase both in the percentage of motile sperm and in the quality (grade) of sperm motility. The data show that hypotaurine is required for expression of sperm motility in the hamster, and support the concept that the loss of hypotaurine from sperm following washing and dilution is responsible for the sperm-immobilizing effect of these procedures. Additionally, the data demonstrate that hamster sperm can remain viable for several hours after becoming immotile, and that many of the immotile sperm are capable of being reactivated. |
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Authors:
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D E Boatman; B D Bavister; E Cruz |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of andrology Volume: 11 ISSN: 0196-3635 ISO Abbreviation: J. Androl. Publication Date: 1990 Jan-Feb |
Date Detail:
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Created Date: 1990-04-25 Completed Date: 1990-04-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8106453 Medline TA: J Androl Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 66-72 Citation Subset: IM |
Affiliation:
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Department of Veterinary Science, University of Wisconsin-Madison 53706. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Cricetinae Drug Synergism Epididymis Epinephrine / pharmacology Male Mesocricetus Penicillamine / pharmacology Sperm Motility / drug effects* Taurine / analogs & derivatives*, pharmacology Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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HD14235/HD/NICHD NIH HHS; RR00167/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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107-35-7/Taurine; 300-84-5/hypotaurine; 51-43-4/Epinephrine; 52-67-5/Penicillamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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