| Addition of glucose enables determination of luciferase activity in carbon-starved, stationary phase Lactococcus lactis cells. | |
| | |
MedLine Citation:
|
PMID: 16904780 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We describe a simple method for measuring luciferase activity in the stationary phase of Lactococcus lactis. Due to large fluctuations in the energy and redox pools of stationary phase bacterial cells, measurement of luciferase activities does not yield reliable results. Upon addition of relatively small amounts of glucose, the pools are restored and measurement of luciferase becomes possible. Since luciferase activities are easily measured, our method allows to apply this simple analytical tool in stationary phase cells. |
| | |
Authors:
|
Bjoern Schuster; Mathias Menzel; Arnold Geis; Knut J Heller |
Publication Detail:
|
Type: Journal Article Date: 2006-08-14 |
Journal Detail:
|
Title: Journal of microbiological methods Volume: 67 ISSN: 0167-7012 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2006 Dec |
Date Detail:
|
Created Date: 2006-10-30 Completed Date: 2007-02-12 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 624-6 Citation Subset: IM |
Affiliation:
|
Institute for Microbiology, Federal Research Centre for Nutrition and Food, Hermann-Weigmann-Str. 1, D-24103 Kiel, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Glucose
/
metabolism* Lactococcus lactis / metabolism* Luciferases / metabolism* |
| Chemical | |
Reg. No./Substance:
|
50-99-7/Glucose; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular monitoring of the intestinal flora by denaturing high performance liquid chromatography.
Next Document: Development of a DNA microarray to identify the Streptococcus pneumoniae serotypes contained in the ...