Document Detail


Addition of glucose enables determination of luciferase activity in carbon-starved, stationary phase Lactococcus lactis cells.
MedLine Citation:
PMID:  16904780     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a simple method for measuring luciferase activity in the stationary phase of Lactococcus lactis. Due to large fluctuations in the energy and redox pools of stationary phase bacterial cells, measurement of luciferase activities does not yield reliable results. Upon addition of relatively small amounts of glucose, the pools are restored and measurement of luciferase becomes possible. Since luciferase activities are easily measured, our method allows to apply this simple analytical tool in stationary phase cells.
Authors:
Bjoern Schuster; Mathias Menzel; Arnold Geis; Knut J Heller
Publication Detail:
Type:  Journal Article     Date:  2006-08-14
Journal Detail:
Title:  Journal of microbiological methods     Volume:  67     ISSN:  0167-7012     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-10-30     Completed Date:  2007-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  624-6     Citation Subset:  IM    
Affiliation:
Institute for Microbiology, Federal Research Centre for Nutrition and Food, Hermann-Weigmann-Str. 1, D-24103 Kiel, Germany.
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MeSH Terms
Descriptor/Qualifier:
Glucose / metabolism*
Lactococcus lactis / metabolism*
Luciferases / metabolism*
Chemical
Reg. No./Substance:
50-99-7/Glucose; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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