| Addition of ascorbate during cryopreservation stimulates subsequent embryo development. | |
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MedLine Citation:
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PMID: 12351549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Embryo development following cryopreservation is reduced compared with fresh embryos. One of the traumas that cryopreservation imparts on embryos is an increase in oxidative stress. Therefore, this study investigated the effects of the addition of the antioxidant ascorbate to the cryopreservation solutions on subsequent embryo development. METHODS: Mouse embryos at the 2-cell and blastocyst stages were either slow-frozen or vitrified in solutions containing either no ascorbate or 0.1 or 0.5 mmol/l ascorbate. The effects on the levels of hydrogen peroxide and subsequent embryo development and physiology were assessed. RESULTS: Addition of ascorbate to the cryopreservation solutions reduced the levels of hydrogen peroxide in embryos. Furthermore, addition of 0.1 mmol/l ascorbate significantly enhanced inner cell mass development in blastocysts. Embryos cryopreserved with ascorbate had significantly lower levels of lactate dehydrogenase leakage, and increased rates of metabolism compared with those cryopreserved in the absence of ascorbate. The benefits of ascorbate were significantly greater in embryos that were slow-frozen compared with those that were vitrified. CONCLUSIONS: These data indicate that the addition of 0.1 mmol/l ascorbate to the cryopreservation solutions for the mammalian embryo would be of significant value. |
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Authors:
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Michelle Lane; Jeffery M Maybach; David K Gardner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 17 ISSN: 0268-1161 ISO Abbreviation: Hum. Reprod. Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-09-27 Completed Date: 2003-03-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 2686-93 Citation Subset: IM |
Affiliation:
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Research Department, Colorado Center for Reproductive Medicine, 799 East Hampden Ave, Suite 520, Englewood 80110, USA. mlane@colocrm.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ascorbic Acid / administration & dosage* Blastocyst / physiology Cryopreservation / methods* Culture Techniques Embryonic and Fetal Development* / drug effects* Female Glucose / metabolism Hydrogen Peroxide / analysis L-Lactate Dehydrogenase / metabolism Mice Mice, Inbred C57BL Mice, Inbred CBA Oxidation-Reduction Pyruvic Acid / metabolism Solutions Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Solutions; 127-17-3/Pyruvic Acid; 50-81-7/Ascorbic Acid; 50-99-7/Glucose; 7722-84-1/Hydrogen Peroxide; EC 1.1.1.27/L-Lactate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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