Document Detail

Adding ROS quenchers to cold K+ cardioplegia reduces superoxide emission during 2-hour global cold cardiac ischemia.
MedLine Citation:
PMID:  21282477     Owner:  NLM     Status:  MEDLINE    
We reported that the combination of reactive oxygen species (ROS) quenchers Mn(III) tetrakis (4-benzoic acid) porphyrin (MnTBAP), catalase, and glutathione (MCG) given before 2 hours cold ischemia better protected cardiac mitochondria against cold ischemia and warm reperfusion (IR)-induced damage than MnTBAP alone. Here, we hypothesize that high K(+) cardioplegia (CP) plus MCG would provide added protection of mitochondrial bioenergetics and cardiac function against IR injury. Using fluorescence spectrophotometry, we monitored redox balance, ie reduced nicotinamide adenine dinucleotide and flavin adenine dinucleotide (NADH/FAD), superoxide (O(2) (•-)), and mitochondrial Ca(2+) (m[Ca(2+)]) in the left ventricular free wall. Guinea pig isolated hearts were perfused with either Krebs Ringer's (KR) solution, CP, or CP + MCG, before and during 27°C perfusion followed immediately by 2 hours of global ischemia at 27°C. Drugs were washed out with KR at the onset of 2 hours 37°C reperfusion. After 120 minutes warm reperfusion, myocardial infarction was lowest in the CP + MCG group and highest in the KR group. Developed left ventricular pressure recovery was similar in CP and CP + MCG and was better than in the KR group. O(2) (•-), m[Ca(2+)], and NADH/FAD were significantly different between the treatment and KR groups. O(2) (•-) was lower in CP + MCG than in the CP group. This study suggests that CP and ROS quenchers act in parallel to improve mitochondrial function and to provide protection against IR injury at 27°C.
Mohammed Aldakkak; David F Stowe; James S Heisner; Matthias L Riess; Amadou K S Camara
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-01-31
Journal Detail:
Title:  Journal of cardiovascular pharmacology and therapeutics     Volume:  17     ISSN:  1940-4034     ISO Abbreviation:  J. Cardiovasc. Pharmacol. Ther.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-07     Completed Date:  2012-10-08     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  9602617     Medline TA:  J Cardiovasc Pharmacol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  93-101     Citation Subset:  IM    
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MeSH Terms
Cardioplegic Solutions / therapeutic use
Cold Ischemia / methods*
Guinea Pigs
Heart Arrest, Induced / methods*
Heart Rate / drug effects,  physiology
Hypertonic Solutions / therapeutic use
Myocardial Ischemia / metabolism,  prevention & control
Myocardial Reperfusion Injury / metabolism*,  prevention & control
Potassium Compounds / therapeutic use*
Random Allocation
Reactive Oxygen Species / antagonists & inhibitors*,  metabolism
Superoxides / antagonists & inhibitors*,  metabolism
Time Factors
Grant Support
Reg. No./Substance:
0/Cardioplegic Solutions; 0/Hypertonic Solutions; 0/Potassium Compounds; 0/Reactive Oxygen Species; 0/potassium cardioplegic solution; 11062-77-4/Superoxides

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