Document Detail

Adaptive stress response to menadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377.
MedLine Citation:
PMID:  22068500     Owner:  NLM     Status:  In-Data-Review    
The molecular mechanisms involved in the ability of yeast cells to adapt and respond to oxidative stress are of great interest to the pharmaceutical, medical, food, and fermentation industries. In this study, we investigated the time-dependent, cellular redox homeostasis ability to adapt to menadione-induced oxidative stress, using biochemical and proteomic approaches in Saccharomyces cerevisiae KNU5377. Time-dependent cell viability was inversely proportional to endogenous amounts of ROS measured by a fluorescence assay with 2',7'-dichlorofluorescin diacetate (DCFHDA), and was hypersensitive when cells were exposed to the compound for 60 min. Morphological changes, protein oxidation and lipid peroxidation were also observed. To overcome the unfavorable conditions due to the presence of menadione, yeast cells activated a variety of cell rescue proteins including antioxidant enzymes, molecular chaperones, energy-generating metabolic enzymes, and antioxidant molecules such as trehalose. Thus, these results show that menadione causes ROS generation and high accumulation of cellular ROS levels, which affects cell viability and cell morphology and there is a correlation between resistance to menadione and the high induction of cell rescue proteins after cells enter into this physiological state, which provides a clue about the complex and dynamic stress response in yeast cells.
Il-Sup Kim; Ho-Yong Sohn; Ingnyol Jin
Publication Detail:
Type:  Journal Article     Date:  2011-11-09
Journal Detail:
Title:  Journal of microbiology (Seoul, Korea)     Volume:  49     ISSN:  1976-3794     ISO Abbreviation:  J. Microbiol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-11-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9703165     Medline TA:  J Microbiol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  816-23     Citation Subset:  IM    
Department of Microbiology, Kyungpook National University, Daegu, 702-701, Republic of Korea,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Biochemical properties of an extracellular trehalase from Malbranchea pulchella var. Sulfurea.
Next Document:  Degradation of endocrine disrupting chemicals by genetic transformants with two lignin degrading enz...