| Adaptive response of equine intestinal Na+/glucose co-transporter (SGLT1) to an increase in dietary soluble carbohydrate. | |
| | |
MedLine Citation:
|
PMID: 19048283 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Experimental and epidemiological evidence suggests that consumption of hydrolyzable carbohydrate, hCHO (grain), by horses is an important risk factor for colic, a common cause of equine mortality. It is unknown whether the small intestinal capacity to digest hCHO and/or to absorb monosaccharides is limiting, or even if horses can adapt to increased carbohydrate load. We investigated changes in the brush-border membrane carbohydrate digestive enzymes and glucose absorptive capacity of horse small intestine in response to increased hCHO. Expression of the Na(+)/glucose co-transporter, SGLT1, was assessed by Western blotting, immunohistochemistry, Northern blotting, QPCR, and Na(+)-dependent D-glucose transport. Glucose transport rates, SGLT1 protein, and mRNA expression were all 2-fold higher in the jejunum and 3- to 5-fold higher in the ileum of horses maintained on a hCHO-enriched diet compared to pasture forage. Activity of the disaccharidases was unaltered by diet. In a well-controlled study, we determined SGLT1 expression in the duodenal and ileal biopsies of horses switched, gradually over a 2-month period, from low (<1.0 g/kg bwt/day) to high hCHO (6.0 g/kg bwt/day) diets of known composition. We show that SGLT1 expression is enhanced, with time, 2-fold in the duodenum and 3.3-fold in the ileum. The study has important implications for dietary management of the horse. |
| | |
Authors:
|
Jane Dyer; Miran Al-Rammahi; Louise Waterfall; Kieron S H Salmon; Ray J Geor; Ludovic Bouré; G Barrie Edwards; Christopher J Proudman; Soraya P Shirazi-Beechey |
Related Documents
:
|
955503 - Relation between body weight and the gastric and intestinal handling of an oral caloric... 7960493 - Effects of type of dietary fat and cholecalciferol on magnesium absorption in rats with... 6119813 - Teratogenicity and embryotoxicity of nickel carbonyl in syrian hamsters. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-02 |
Journal Detail:
|
Title: Pflügers Archiv : European journal of physiology Volume: 458 ISSN: 1432-2013 ISO Abbreviation: Pflugers Arch. Publication Date: 2009 Jun |
Date Detail:
|
Created Date: 2009-05-13 Completed Date: 2009-08-18 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: Germany |
Other Details:
|
Languages: eng Pagination: 419-30 Citation Subset: IM |
Affiliation:
|
Epithelial Function and Development Group, Department of Veterinary Preclinical Sciences, The University of Liverpool, Brownlow Hill and Crown Street, Liverpool L69 7ZJ, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adaptation, Physiological Animals Dietary Carbohydrates / pharmacology* Female Glucose Transporter Type 2 / biosynthesis Horses Ileum / drug effects, metabolism Immunohistochemistry Jejunum / drug effects, metabolism Male Microvilli / metabolism RNA, Messenger / metabolism Sodium-Glucose Transporter 1 / biosynthesis, metabolism* Solubility Sucrase / metabolism alpha-Glucosidases / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Dietary Carbohydrates; 0/Glucose Transporter Type 2; 0/RNA, Messenger; 0/Sodium-Glucose Transporter 1; EC 3.2.1.20/alpha-Glucosidases; EC 3.2.1.48/Sucrase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Estrogen and raloxifene improve metaphyseal fracture healing in the early phase of osteoporosis. A n...
Next Document: Pairwise coupling of hair cell transducer channels links auditory sensitivity and dynamic range.