| Adaptive force sonorheometry for assessment of whole blood coagulation. | |
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MedLine Citation:
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PMID: 20096680 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Viscoelastic diagnostics that monitor the hemostatic function of whole blood (WB), such as thromboelastography, have been developed with demonstrated clinical utility. By measuring the cumulative effects of all components of hemostasis, viscoelastic diagnostics have circumvented many of the challenges associated with more common tests of blood coagulation. METHODS: We describe a new technology, called sonorheometry, that adaptively applies acoustic radiation force to assess coagulation function in WB. The repeatability (precision) of coagulation parameters was assessed using citrated WB samples. A reference range of coagulation parameters, along with corresponding measurements from prothrombin time (PT) and partial thromboplastin time (PTT), were obtained from WB samples of 20 healthy volunteers. In another study, sonorheometry monitored anticoagulation with heparin (0-5 IU/ml) and reversal from varied dosages of protamine (0-10 IU/ml) in heparinized WB (2 IU/ml). RESULTS: Sonorheometry exhibited low CVs for parameters: clot initiation time (TC1), <7%; clot stabilization time (TC2), <6.5%; and clotting angle (theta), <3.5%. Good correlation was observed between clotting times, TC1 and TC2, and PTT (r=0.65 and 0.74 respectively; n=18). Linearity to heparin dosage was observed with average linearity r>0.98 for all coagulation parameters. We observed maximum reversal of heparin anticoagulation at protamine to heparin ratios of 1.4:1 from TC1 (P=0.6) and 1.2:1 from theta (P=0.55). CONCLUSIONS: Sonorheometry is a non-contact method for precise assessment of WB coagulation. |
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Authors:
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F William Mauldin; Francesco Viola; Theresa C Hamer; Eman M Ahmed; Shawna B Crawford; Doris M Haverstick; Michael B Lawrence; William F Walker |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-01-22 |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 411 ISSN: 1873-3492 ISO Abbreviation: Clin. Chim. Acta Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-03-29 Completed Date: 2010-07-27 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 638-44 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blood Coagulation / drug effects Blood Coagulation Tests / instrumentation, methods* Female Heparin / pharmacology Humans Male Partial Thromboplastin Time Protamines / pharmacology Prothrombin Time Rheology / instrumentation, methods* Signal Processing, Computer-Assisted Transducers Ultrasonics Viscoelastic Substances / chemistry Whole Blood Coagulation Time Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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R01 EB002185-09/EB/NIBIB NIH HHS; R01 EB005433-01/EB/NIBIB NIH HHS; R01 EB005433-04/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Protamines; 0/Viscoelastic Substances; 9005-49-6/Heparin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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