Document Detail

Adaptive evolution of Corynebacterium glutamicum resistant to oxidative stress and its global gene expression profiling.
MedLine Citation:
PMID:  23288296     Owner:  NLM     Status:  Publisher    
Corynebacterium glutamicum was adapted in a chemostat for 1,900 h with gradually increasing H(2)O(2) stress to understand the oxidative stress response of an industrial host. After 411 generations of adaptation, C. glutamicum developed the ability to grow under stress of 10 mM H(2)O(2), whereas the wild-type did not. The adapted strain also showed increased stress resistance to diamide and menadione, SDS, Tween 20, HCl, NaOH, and ampicillin. A total of 1,180 genes in the RNA-seq transcriptome analysis of the adapted strain were up-regulated twice or higher (corresponding to 38.6 % of the genome), and 126 genes were down-regulated half or less (4.1 % of genome) under 10 mM H(2)O(2)-stress conditions compared with those of the wild-type under a no stress condition. Especially the aromatic compound-degrading gene clusters (vanRABK, pcaJIRFLO, and benABCDRKE) were more than threefold up-regulated. Plausible reasons for the H(2)O(2)-stress tolerance of the adapted strain are discussed as well as the potential strategy for development of oxidative stress-tolerant strain.
Joo-Young Lee; Jiyoon Seo; Eung-Soo Kim; Heung-Shick Lee; Pil Kim
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-4
Journal Detail:
Title:  Biotechnology letters     Volume:  -     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi, 420-743, South Korea.
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