Document Detail


Adapted liquid crystal display backlighting unit for densitometry of stained polyacrylamide electrophoresis gels.
MedLine Citation:
PMID:  19309016     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Appropriate monochromatic illumination that is spatially uniform will ensure densitometry of stained polyacrylamide electrophoresis gels with high sensitivity and repeatability. The approach of using an array of LEDs as an illuminator has practical limitations. Here we describe an alternative method based on adapted liquid crystal display backlighting. By changing the fluorescent light source and using a region where light intensity is uniform, we demonstrate the ability to achieve highly sensitive and repeatable densitometry measurements of polyacrylamide electrophoresis gels with Coomassie-stained proteins.
Authors:
Han Yen Tan; Tuck Wah Ng; Oi Wah Liew
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  30     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-04-01     Completed Date:  2009-08-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  987-90     Citation Subset:  IM    
Affiliation:
Department of Mechanical & Aerospace Engineering, Monash University, VIC, Australia.
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MeSH Terms
Descriptor/Qualifier:
Densitometry / instrumentation*
Electrophoresis, Polyacrylamide Gel*
Lighting*
Linear Models
Liquid Crystals
Reproducibility of Results
Rosaniline Dyes / chemistry
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Rosaniline Dyes; 78642-64-5/Coomassie blue

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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