Document Detail


Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.).
MedLine Citation:
PMID:  23038737     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In addition to high drinking rates, processing of intestinal fluid is essential for osmoregulation in marine fish. This study analyzed the long-term response of the sea bream (Sparus aurata L.) to relevant changes of external salinity 12, 35 and 55ppt focusing on the anterior intestine and in the less often studied rectum. Intestinal water absorption, epithelial HCO(3)(-) secretion and gene expression of the main molecular mechanisms (SLC26a6, SLC26a3, SLC4a4, atp6v1b, CFTR, NKCC1 and NKCC2) involved in Cl- and HCO(3)(-) movements were examined. The anion transporters SLC26a6 and SLC26a3 are several fold more expressed in the anterior intestine, while the expression of Atp6v1b (V-type H(+)-ATPase β subunit) several fold higher in the rectum. Prolonged exposure to altered external salinity was without effect on water absorption but associated with concomitant changes in intestinal fluid content, epithelial HCO(3)(-) secretion and salinity dependent expression of SLC26a6, SLC26a3 and SLC4a4 in the anterior intestine. However, the most striking response to external salinity was obtained in the rectum, where a 4-5-fold increase in water absorption was paralleled by a 2-3-fold increase in HCO(3)(-) secretion in response to 55 ppt. In addition, the rectum of high salinity acclimated fish shows a sustained (and enhanced) secretory current (Isc) identified in vitro in Ussing chambers, confirmed by the higher expression CFTR and NKCC1 and established by protein localization with immunohistochemistry. Taken together the present results suggest a functional anterior-posterior specialization with regards to intestinal fluid processing and subsequently to salinity adaptation of the sea bream. The rectum becomes more active at higher salinities and functions as the final controller of the intestinal function in osmoregulation.
Authors:
Sílvia F Gregório; Edison S M Carvalho; Sandra Encarnação; Jonathan M Wilson; Deborah M Power; Adelino V M Canário; Juan Fuentes
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  The Journal of experimental biology     Volume:  -     ISSN:  1477-9145     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
CCMar, Universidade do Algarve, Portugal;
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