Document Detail

Adaptation-promoting effect of IP3, Ca2+, and phorbol ester on the sugar taste receptor cell of the blowfly, Phormia regina.
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MedLine Citation:
PMID:  1474374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fly has a receptor cell highly specialized for the taste of sugars. We introduced inositol 1,4,5-trisphosphate (IP3), Ca2+, or a phorbol ester, 12-deoxyphorbol 13-isobutylate 20-acetate (DPBA), into the cell and investigated their effects on the response to sucrose. The sugar receptor cell generates impulses during constant stimulation with sucrose, but the impulse frequency gradually declines as the cell adapts to the stimulus. Thus, this gradual reduction of the impulse frequency is a direct manifestation of adaptation of the cell. These reagents accelerated the gradual reduction of the impulse frequency, although the initial impulse frequency was little affected. In contrast to these reagents, glycoletherdiamine-tetraacetate (EGTA) retarded the gradual reduction of the impulse frequency. Moreover, when IP3 and DPBA were applied together, the gradual reduction of the impulse frequency was more accelerated than when either IP3 or DPBA was applied. When IP3 and EGTA were applied together, however, the accelerating effect of IP3 tended to be canceled. Based on these results, we hypothesized that an intracellular cascade involving inositol phospholipid hydrolysis, intracellular Ca2+ mobilization, and protein kinase C-mediated phosphorylation promotes adaptation of the sugar receptor cell.
Authors:
M Ozaki; T Amakawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general physiology     Volume:  100     ISSN:  0022-1295     ISO Abbreviation:  J. Gen. Physiol.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-02-01     Completed Date:  1993-02-01     Revised Date:  2008-11-20    
Medline Journal Info:
Nlm Unique ID:  2985110R     Medline TA:  J Gen Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  867-79     Citation Subset:  IM    
Affiliation:
Department of Biology, College of General Education, Kobe University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / drug effects
Animals
Calcium / pharmacology
Diptera / physiology*
Egtazic Acid / pharmacology
Electrophysiology
Inositol 1,4,5-Trisphosphate / pharmacology
Models, Biological
Phorbol Esters / pharmacology
Sucrose / pharmacology
Taste / drug effects*,  physiology
Taste Buds / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Phorbol Esters; 25090-71-5/12-deoxyphorbol-13-isobutyrate-20-acetate; 57-50-1/Sucrose; 67-42-5/Egtazic Acid; 7440-70-2/Calcium; 85166-31-0/Inositol 1,4,5-Trisphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Gen Physiol
ISSN: 0022-1295
ISSN: 1540-7748
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 11 Year: 1992
Volume: 100 Issue: 5
First Page: 867 Last Page: 879
ID: 2229116
Publisher Id: 93115699
PubMed Id: 1474374

Adaptation-promoting effect of IP3, Ca2+, and phorbol ester on the sugar taste receptor cell of the blowfly, Phormia regina


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