Document Detail


Adaptation of canine femoral head articular cartilage to long distance running exercise in young beagles.
MedLine Citation:
PMID:  8323385     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To study the effects of long term (one year), long distance (up to 40 km/day) running on the metabolism of articular cartilage the biosynthesis of proteoglycans was examined by in vitro labelling of anterior (weight bearing) and posterior (less weight bearing) areas of the femoral head from young beagles. METHODS: Total sulphate incorporation rates were determined and distribution of the incorporated sulphate was localised by quantitative autoradiography. Concentration and extractability of the proteoglycans were determined, and proteoglycan structures were investigated by gel filtration chromatography, agarose gel electrophoresis, and chemical determinations. RESULTS: In the less weight bearing area the amount of extractable proteoglycans was decreased (p < or = 0.02), simultaneously with an increased concentration of residual glycosaminoglycans in the tissue after 4 M GuCl extraction (p < or = 0.05). In control animals proteoglycan synthesis was most active in the deep zone of the cartilage, whereas exercise increased synthesis in the intermediate zone. There was a tendency to a lower keratan: chondroitin sulphate ratio in the running dogs. No macroscopical or microscopical signs of articular degeneration or injury were visible in any of the animals. CONCLUSION: The articular cartilage of the femoral head showed a great capacity to adapt to the increased mechanical loading. The reduced proteoglycan extractability in the less weight bearing area changed it similar to the weight bearing area, suggesting that the low extractability of proteoglycans reflects the long term loading history of articular cartilage. The congruency of the femoral head with acetabulum seems to protect the cartilage from the untoward alterations that occur in the femoral condyles subjected to a similar running programme.
Authors:
M J Lammi; T P H?kkinen; J J Parkkinen; M M Hyttinen; M Jortikka; H J Helminen; M I Tammi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of the rheumatic diseases     Volume:  52     ISSN:  0003-4967     ISO Abbreviation:  Ann. Rheum. Dis.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-08-03     Completed Date:  1993-08-03     Revised Date:  2010-03-24    
Medline Journal Info:
Nlm Unique ID:  0372355     Medline TA:  Ann Rheum Dis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  369-77     Citation Subset:  IM; S    
Affiliation:
Department of Anatomy, University of Kuopio, Finland.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / physiology*
Animals
Cartilage, Articular / metabolism,  physiology*
Chromatography, Gel
Culture Techniques
Dogs
Electrophoresis, Polyacrylamide Gel
Female
Femur Head / physiology*
Proteoglycans / metabolism
Running / physiology*
Sulfates / metabolism
Weight-Bearing / physiology
Chemical
Reg. No./Substance:
0/Proteoglycans; 0/Sulfates
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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