Document Detail


Adaptation of an automated microbiology system for the growth of anaerobes and performance of antimicrobial susceptibility tests.
MedLine Citation:
PMID:  6291376     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The authors examined the feasibility of growing anaerobes in an automated microbiology system (MS-2) and using the system for antimicrobial susceptibility testing. A total of 78 of 100 clinical anaerobic isolates grew in the MS-2 if 100 microL of an undiluted overnight suspension of organisms was inoculated into at least 2.5 mL of freshly prepared Wilkins-Chalgren broth supplemented with 0.07% agar. Susceptibility test results were determined with 50 anaerobes tested against seven antibiotics in the MS-2 system and were compared with results determined with a qualitative reference susceptibility test method. of the 350 tests, 88% of the MS-2 results agreed with the qualitative reference results. False-susceptible and false-resistant results were reported for 8.6 and 3.4%, respectively, of the MS-2 results.
Authors:
P R Murray; A C Niles
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  78     ISSN:  0002-9173     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1982-12-16     Completed Date:  1982-12-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  457-61     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Actinomyces / growth & development
Anaerobiosis
Bacteriological Techniques*
Bacteroides fragilis / growth & development*
Clostridium perfringens / growth & development*
Computers
Culture Media
Humans
Incubators
Microbial Sensitivity Tests / methods
Peptococcus / growth & development*
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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