Document Detail


Acyltransferases and transacylases involved in fatty acid remodeling of phospholipids and metabolism of bioactive lipids in mammalian cells.
MedLine Citation:
PMID:  9276665     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Over 100 different phospholipid molecular species are known to be present in mammalian cells and tissues. Fatty acid remodeling systems for phospholipids including acyl-CoA: lysophospholipid acyltransferases, CoA-dependent and CoA-independent transacylation systems and lysophospholipase/transacylase are involved in the biosynthesis of these molecular species. Acyl-CoA:1-acyl-2-lysophospholipid acyltransferase prefers polyunsaturated fatty acyl-CoAs as acyl donors while acyl-CoA:2-acyl-1-lysophospholipid acyltransferase prefers saturated fatty acyl-CoAs. Therefore, the acyl-CoA:lysophospholipid acyltransferase system is involved in the synthesis of the phospholipid molecular species containing sn-1 saturated and sn-2 unsaturated fatty acids. The CoA-dependent transacylation system catalyzes the transfer of fatty acids esterified in phospholipids to lysophospholipids in the presence of CoA without the generation of free fatty acids. The CoA-dependent transacylation reaction in rat liver exhibits strict fatty acid specificity, i.e., three types of fatty acids (20:4, 18:2, and 18:0) are transferred. On the other hand, the CoA-independent transacylase catalyzes the transfer of C20 and C22 polyunsaturated fatty acids from diacyl phospholipids to various lysophospholipids, in particular, ether-containing lysophospholipids, in the absence of any cofactors. The CoA-independent transacylase is assumed to be involved in the accumulation of polyunsaturated fatty acids in ether-containing phospholipids and in the removal of deleterious ether-containing lysophospholipids. These acyltransferases and transacylases are involved in not only the remodeling of fatty acids but also the synthesis and degradation of some bioactive lipids and their precursors. In this review, the properties of these fatty acid remodeling systems and their possible roles in the biosynthesis of bioactive lipids are described.
Authors:
A Yamashita; T Sugiura; K Waku
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Journal of biochemistry     Volume:  122     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-10-06     Completed Date:  1997-10-06     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1-16     Citation Subset:  IM    
Affiliation:
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa. ayamashi@pharm.teikyo-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
1-Acylglycerophosphocholine O-Acyltransferase / metabolism
Acyltransferases / metabolism*
Animals
Arachidonic Acid / metabolism
Fatty Acids / metabolism*
Humans
Lipid Metabolism*
Lysophospholipase / metabolism
Mammals / metabolism*
Phospholipases A / metabolism
Phospholipids / chemistry,  metabolism
Platelet Activating Factor / biosynthesis
Rats
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Phospholipids; 0/Platelet Activating Factor; 506-32-1/Arachidonic Acid; EC 2.3.-/Acyltransferases; EC 2.3.1.23/1-Acylglycerophosphocholine O-Acyltransferase; EC 3.1.1.-/Phospholipases A; EC 3.1.1.5/Lysophospholipase

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