Document Detail


Acylation of bovine rhodopsin by [3H]palmitic acid.
MedLine Citation:
PMID:  6715336     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bovine retinas or preparations of rod outer segments incorporate [3H]palmitic acid into rhodopsin. The incorporation is both time- and temperature-dependent. The major product retains the chromatographic and electrophoretic properties of rhodopsin and remains photosensitive as demonstrated by alteration of its chromatographic behavior upon exposure to light. The incorporated radioactivity resists extraction with organic solvents and is not dissociated from the protein by detergents or under the denaturing conditions of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Radioactive free fatty acid can, however, be released by alkaline hydrolysis. Hydroxylamine treatment yields a mixture of the free fatty acid and the fatty acyl hydroxamate. These results demonstrate the formation of an ester bond between [3H]palmitic acid and rhodopsin. Cycloheximide fails to inhibit the incorporation. This finding along with the ability of rod outer segments to support the incorporation point to the acylation of rhodopsin as a late post-translational event.
Authors:
P J O'Brien; M Zatz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  259     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-05-30     Completed Date:  1984-05-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5054-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acylation
Animals
Cattle
Palmitic Acids / metabolism*
Retina / metabolism*
Retinal Pigments / metabolism*
Rhodopsin / isolation & purification,  metabolism*
Rod Cell Outer Segment / metabolism
Tritium / diagnostic use
Chemical
Reg. No./Substance:
0/Palmitic Acids; 0/Retinal Pigments; 10028-17-8/Tritium; 9009-81-8/Rhodopsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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