Document Detail


Acyl moieties modulate the effects of phospholipids on beta-carotene uptake by Caco-2 cells.
MedLine Citation:
PMID:  17069346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The intestinal absorption of carotenoids is thought to be mediated by the carotenoid assembly in mixed micelles, followed by its transfer into the enterocytes and subsequent secretion to the lymph as chylomicron particles. In the present study we investigated the effects of phospholipids and lysophospholipids with diverse fatty acyl moieties on the uptake of beta-carotene solubilized in mixed micelles by Caco-2 cells. Compared with phospholipid-free mixed micelles (NoPL), those containing long-chain PC inhibited beta-carotene uptake (16:0,18:1-PC approximately equal to 16:0,18:2-PC < 14:0,14:0-PC approximately equal to 16:0, 14:0-PC < 16:0,16:0-PC < NoPL). However, mixed micelles containing medium-chain PC enhanced beta-carotene uptake (NoPL < 8:0,8:0-PC < 12:0,12:0-PC < 10:0,10:0-PC), and short-chain PC did not affect the uptake. Among the lysophosphatidylcholine (LysoPC) class, a marked increase of beta-carotene uptake by medium-to-long-chain LysoPC was observed (NoPL < 12:0-LysoPC < 14:0-LysoPC < 18:1-LysoPC < 16:0-LysoPC), although short-to-medium-chain LysoPC (6:0-LysoPC to 10:0-LysoPC) did not affect beta-carotene uptake. The long-chain 16:0,18:1-PC increased the beta-carotene efflux from cells and drastically changed the beta-carotene UV-visible absorbance spectrum, compared with those of NoPL micelles. The acyl moieties of long-chain PC may interact with the carotenoid in the micelle interior, shifting the beta-carotene partition toward the micellar phase. Medium-chain PC and long-chain LysoPC, which have nearly equivalent hydrophobicities, may enhance beta-carotene uptake through their interaction with the cell membrane.
Authors:
Lina Yonekura; Wakako Tsuzuki; Akihiko Nagao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  41     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-10-30     Completed Date:  2006-11-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  629-36     Citation Subset:  IM    
Affiliation:
National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport / drug effects
Caco-2 Cells
Fatty Acids / chemistry,  metabolism*
Humans
Intestinal Absorption / physiology
Lysophosphatidylcholines / chemistry,  metabolism
Micelles
Phospholipids / chemistry,  metabolism*,  pharmacology
beta Carotene / pharmacokinetics*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Lysophosphatidylcholines; 0/Micelles; 0/Phospholipids; 7235-40-7/beta Carotene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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