| Acyl-CoA synthetase activity of brown adipose tissue mitochondria. Substrate specificity and its relation to the endogenous pool of long-chain fatty acids. | |
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MedLine Citation:
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PMID: 6106505 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. A new assay of acyl-CoA synthetase (acid:CoA ligase (AMP-forming), EC 6.2.1.3) activity was developed for application to measurements on isolated intact mitochondria. The assay was based on the formation of the product AMP as determined by high-performance liquid chromatography (HPLC), making corrections for the partial conversion of AMP to ADP by the adenylate kinase reaction. 2. The substrate specificity of the long-chain acyl-CoA synthetase was measured in brown adipose tissue mitochondria, isolated from cold-acclimated guinea-pigs. Using mitochondria largely deficient of endogenous fatty acids, the synthetase activity (V') revealed an optimum at 12 :0 and 13 : 0 with saturated fatty acids as the substrate. In contrast to other tissues, the highest V' values were observed with unsaturated fatty acids 18 : 1 (9) (cis) and 18 : 2 (9, 12) (all cis). The apparent Michaelis constant varied within a narrow range and revealed no systematic dependence on the chain-length as was the case for V'. Since the mitochondria have a high capacity of fatty acid binding, with unknown affinity, the estimated K'm values are, however, of questionable significance. 3. The pattern of chain-length specificity in the synthetase reaction of the mitochondria compares well with the pattern of endogenous long-chain fatty acids in which mainly four species contribute, i.e. 18 : 1 (42 mol%) and 18 : 2 (18 mol%), 16 : 0 (26 mol%) and 18 : 0 (14 mol%). |
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Authors:
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P T Normann; T Flatmark |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 619 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1980 Jul |
Date Detail:
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Created Date: 1980-12-18 Completed Date: 1980-12-18 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1-10 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetate-CoA Ligase
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metabolism* Adenosine Monophosphate / biosynthesis Adenosine Triphosphate / metabolism Adipose Tissue, Brown / enzymology* Animals Coenzyme A Ligases / metabolism* Cold Temperature Fatty Acids / metabolism Guinea Pigs Hydrogen-Ion Concentration Kinetics Mitochondria / enzymology* Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 56-65-5/Adenosine Triphosphate; 61-19-8/Adenosine Monophosphate; EC 6.2.1.-/Coenzyme A Ligases; EC 6.2.1.1/Acetate-CoA Ligase |
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