Document Detail

Acyl-CoA synthetase activity of brown adipose tissue mitochondria. Substrate specificity and its relation to the endogenous pool of long-chain fatty acids.
MedLine Citation:
PMID:  6106505     Owner:  NLM     Status:  MEDLINE    
1. A new assay of acyl-CoA synthetase (acid:CoA ligase (AMP-forming), EC activity was developed for application to measurements on isolated intact mitochondria. The assay was based on the formation of the product AMP as determined by high-performance liquid chromatography (HPLC), making corrections for the partial conversion of AMP to ADP by the adenylate kinase reaction. 2. The substrate specificity of the long-chain acyl-CoA synthetase was measured in brown adipose tissue mitochondria, isolated from cold-acclimated guinea-pigs. Using mitochondria largely deficient of endogenous fatty acids, the synthetase activity (V') revealed an optimum at 12 :0 and 13 : 0 with saturated fatty acids as the substrate. In contrast to other tissues, the highest V' values were observed with unsaturated fatty acids 18 : 1 (9) (cis) and 18 : 2 (9, 12) (all cis). The apparent Michaelis constant varied within a narrow range and revealed no systematic dependence on the chain-length as was the case for V'. Since the mitochondria have a high capacity of fatty acid binding, with unknown affinity, the estimated K'm values are, however, of questionable significance. 3. The pattern of chain-length specificity in the synthetase reaction of the mitochondria compares well with the pattern of endogenous long-chain fatty acids in which mainly four species contribute, i.e. 18 : 1 (42 mol%) and 18 : 2 (18 mol%), 16 : 0 (26 mol%) and 18 : 0 (14 mol%).
P T Normann; T Flatmark
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  619     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1980 Jul 
Date Detail:
Created Date:  1980-12-18     Completed Date:  1980-12-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
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MeSH Terms
Acetate-CoA Ligase / metabolism*
Adenosine Monophosphate / biosynthesis
Adenosine Triphosphate / metabolism
Adipose Tissue, Brown / enzymology*
Coenzyme A Ligases / metabolism*
Cold Temperature
Fatty Acids / metabolism
Guinea Pigs
Hydrogen-Ion Concentration
Mitochondria / enzymology*
Substrate Specificity
Reg. No./Substance:
0/Fatty Acids; 56-65-5/Adenosine Triphosphate; 61-19-8/Adenosine Monophosphate; EC 6.2.1.-/Coenzyme A Ligases; EC Ligase

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