| Acute venous occlusion enhances matrix metalloprotease activity: Implications on endothelial dysfunction. | |
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MedLine Citation:
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PMID: 20923679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Venous hypertension is associated with microvascular inflammation, restructuring, and apoptosis, but the cellular and molecular mechanisms underlying these events remain uncertain. In the present study, we tested the hypothesis that elevated venous pressure and reduction of shear stress induce elevated enzymatic activity. This activity in turn may affect endothelial surface receptors and promote their dysfunction. Using a rodent model for venous hypertension using acute venular occlusion, microzymographic techniques for enzyme detection, and immunohistochemistry for receptor labeling, we found increased activity of the matrix metalloproteases (MMPs) -1, -8, and -9 and tissue inhibitors of metalloproteases (TIMPs) -1 and -2 in both high- and low-pressure regions. In this short time frame, we also observed that elevated venule pressure led to two different fates for the vascular endothelial growth factor receptor-2 (VEGFR2); in higher-pressure upstream regions, some animals exhibited higher VEGFR2 expression, while others displayed lower levels upstream compared to their downstream counterparts with lower pressure. VEGFR2 expression was, on average, more pronounced upon application of MMP inhibitor, suggesting possible cleavage of the receptor by activated enzymes in this model. We conclude that venous pressure elevation increases enzymatic activity which may contribute to inflammation and endothelial dysfunction associated with this disease by influencing critical surface receptors. |
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Authors:
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Tom Alsaigh; Elizabeth S Pocock; John J Bergan; Geert W Schmid-Schönbein |
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Publication Detail:
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Type: Journal Article Date: 2010-10-16 |
Journal Detail:
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Title: Microvascular research Volume: 81 ISSN: 1095-9319 ISO Abbreviation: Microvasc. Res. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-10 Completed Date: 2011-05-27 Revised Date: 2012-01-04 |
Medline Journal Info:
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Nlm Unique ID: 0165035 Medline TA: Microvasc Res Country: United States |
Other Details:
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Languages: eng Pagination: 108-16 Citation Subset: IM |
Copyright Information:
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2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Bioengineering, The Institute for Engineering in Medicine, University of California San Diego, La Jolla, CA 92093-0412, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocatalysis / drug effects Dipeptides / pharmacology Endothelial Cells / enzymology, metabolism Endothelium, Vascular / enzymology*, metabolism, physiopathology* Hypertension / enzymology, metabolism, physiopathology Leukocytes / enzymology Male Matrix Metalloproteinase 1 / antagonists & inhibitors, metabolism Matrix Metalloproteinase 2 / metabolism Matrix Metalloproteinase 3 / metabolism Matrix Metalloproteinase 8 / antagonists & inhibitors, metabolism Matrix Metalloproteinase 9 / antagonists & inhibitors, metabolism Matrix Metalloproteinases / metabolism* Mesenteric Vascular Occlusion / enzymology*, metabolism, physiopathology Mesenteric Veins / enzymology*, metabolism, physiopathology Rats Rats, Wistar Reperfusion Tissue Inhibitor of Metalloproteinase-1 / metabolism Tissue Inhibitor of Metalloproteinase-2 / metabolism Vascular Endothelial Growth Factor Receptor-2 / metabolism Venules / enzymology, metabolism, physiopathology |
| Grant Support | |
ID/Acronym/Agency:
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R01 HL010881-41/HL/NHLBI NIH HHS; R01 HL010881-42/HL/NHLBI NIH HHS; R01 HL010881-43/HL/NHLBI NIH HHS; R01 HL010881-44/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Dipeptides; 0/GM 6001; 0/Tissue Inhibitor of Metalloproteinase-1; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-2; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.17/Matrix Metalloproteinase 3; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.34/Matrix Metalloproteinase 8; EC 3.4.24.35/Matrix Metalloproteinase 9; EC 3.4.24.7/Matrix Metalloproteinase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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