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Acute and subacute toxicity of novel biogenic selenium nanoparticles in mice.
MedLine Citation:
PMID:  23035822     Owner:  NLM     Status:  Publisher    
Context: In the present investigation, acute and subacute toxicity of the biogenic Se nanoparticles (Se NPs) has been reported. Objective: To characterize the Se NPs produced by a bacterium species and to evaluate their toxicity and impact on clinical chemistry and hematological parameters of NMRI mice. Materials and methods: The Se NPs were prepared by Bacillus sp. MSh-1 in a culture medium containing SeO(2) (1.26 mM) and their physiochemical properties investigated using TEM, XRD and FT-IR. The LD(50) of Se NPs and SeO(2) were determined and the subacute toxicity evaluated by orally administration of 0, 2.5, 5, 10 and 20 mg kg(-1) of Se NPs to male mice for 14 consecutive days. Parameters of blood cells, AST, ALT, ALP, creatinine, BUN, cholesterol, bilirubin, triglyceride and CPK were experimentally measured. Results: The XRD and TEM analyses showed that the spherical NPs were amorphous, in the size range of 80-220 nm. The toxicological evaluation showed that the LD(50) values of SeO(2) and Se NPs were 7.3 and 198.1 mg kg(-1), respectively. No biochemical changes were observed from the administration of 2.5, 5 and 10 mg kg(-1) of Se NPs, but a dose of 20 mg kg(-1) was accompanied with signs of toxicity including lower body weight and changes in clinical chemistry and hematological parameters. Conclusion: The biogenic Se NPs were less toxic than synthetic Se NPs and much less (26-fold) toxic than the SeO(2), which demonstrates the important role of Bacillus sp. MSh-1 in conversion of a highly toxic Se compound to the less toxic Se NPs.
Mojtaba Shakibaie; Ahmad Reza Shahverdi; Mohammad Ali Faramarzi; Gholam Reza Hassanzadeh; Hamid Reza Rahimi; Omid Sabzevari
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Pharmaceutical biology     Volume:  -     ISSN:  1744-5116     ISO Abbreviation:  Pharm Biol     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9812552     Medline TA:  Pharm Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Pharmaceutics Research Center, Kerman University of Medical Sciences , Kerman , Iran.
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