Document Detail

Acute secretory cell toxicity and epithelial exfoliation after smoke inhalation injury in sheep: an electron and light microscopic study.
MedLine Citation:
PMID:  20843269     Owner:  NLM     Status:  MEDLINE    
Smoke inhalation injury promotes exfoliation of the upper airway columnar epithelium. Tracheal tissues from sheep 30 min after smoke exposure show intact epithelial areas, areas of epithelial disruption with loss of columnar cells and areas denuded of columnar cells. In intact areas detaching ciliated cells can be seen raised above the apical surface. This study aims to assess cell-specific toxicity by examining intact epithelium after inhalation injury. The junctional adhesion integrity between columnar and basal cells and the type of cells initially being displaced were also studied using light (LM) and transmission electron microscopy (TEM). TEM assessment of intact areas of sheep tracheal tissue (n=3) 30 min after exposure showed secretory cell toxicity including extrusion of cytoplasmic contents. In cells with severe secretory cell cytoplasmic disruption, loss of desmosomal junctions between the secretory and adjacent ciliated cells was evident. The number of desmosomes visible between columnar cells and basal cells was reduced (2.8 ± 1.8) in smoke-exposed animals compared to those in uninjured animals (5.0 ± 2.7), p=0.008. Serial sections of intact regions found 52 cells being displaced from the epithelium. All detaching cells were identified as ciliated cells. These studies show that the acute effects of inhalation injury include selective secretory cell toxicity which is associated with loss of junctional adhesion mechanisms and displacement of ciliated cells. Improved understanding of acute hypersecretory responses and epithelial integrity after exposure to toxic agents may improve understanding of epithelial fragility in airway disease.
Sam Jacob; Robert Kraft; Yong Zhu; Reuben K Jacob; David N Herndon; Daniel L Traber; Hal K Hawkins; Robert A Cox
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicology mechanisms and methods     Volume:  20     ISSN:  1537-6524     ISO Abbreviation:  Toxicol. Mech. Methods     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-16     Completed Date:  2010-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134521     Medline TA:  Toxicol Mech Methods     Country:  England    
Other Details:
Languages:  eng     Pagination:  504-9     Citation Subset:  IM    
Shriners Hospital for Children, University of Texas Medical Branch, Galveston, Texas 77555, USA.
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MeSH Terms
Cilia / drug effects,  ultrastructure
Desmosomes / drug effects,  ultrastructure
Disease Models, Animal
Exocrine Glands / drug effects,  ultrastructure
Inhalation Exposure / adverse effects*
Microscopy, Electron, Transmission
Respiratory Mucosa / drug effects*,  ultrastructure
Sheep / physiology*
Smoke / adverse effects*
Smoke Inhalation Injury / pathology*
Trachea / drug effects*,  ultrastructure
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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