Document Detail

Acute and reversible fatty metamorphosis of cultured rat hepatocytes.
MedLine Citation:
PMID:  9088028     Owner:  NLM     Status:  MEDLINE    
Rat hepatocyte cell line M6, B347 and J525, among which only M6 is slightly deviated from diploidy, showed marked microvesicular fatty metamorphosis in response to treatment with Tweens at concentrations of 0.05-0.025% in Eagle's minimum essential medium (MEM). Within 24 h treated cells became fatty at 100% in frequency and filled with small lipid droplets, as revealed by fat staining or at the ultrastructural level. Fatty hepatocytes, however, took again non-fatty morphology 72 h after withdrawal of Tweens from the culture medium. Growth of the cell exhibited mild retardation at the early phase of the treatment but almost similar cell density to that of control cells was achieved 24 h after the treatment. Other detergents without fatty-acid moiety, including NP-40, triton X-100, sodium deoxycholic acid and sodium cholic acid, were ineffective to induce fatty change. Oleate, a fatty-acid moiety of Tween 80 or 85, and linolate caused reversible fatty metamorphosis of the cell lines at concentrations of 1.9 x 10(4) mol/L or more and 3.8 x 10(4) mol/L or more, respectively. Ethanol induced mild steatosis of the cell lines and enhanced fatty change by linolate. Hepatic fatty acid-binding protein was not detected in the cell lines before or after the induction of fatty change. These results indicate that fatty acid itself is directly incorporated in cultured rat hepatocytes and expelled 3 days later without apparent cell degeneration.
T Yamaki; Y Tsu-ura; K Watanabe; T Fukuda; T Suzuki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pathology international     Volume:  47     ISSN:  1320-5463     ISO Abbreviation:  Pathol. Int.     Publication Date:    1997 Feb-Mar
Date Detail:
Created Date:  1997-07-01     Completed Date:  1997-07-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9431380     Medline TA:  Pathol Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  103-11     Citation Subset:  IM    
Department of Pathology, Fukushima Medical College, Japan.
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MeSH Terms
Bucladesine / pharmacology
Carrier Proteins / metabolism
Cell Division / drug effects
Cell Size / drug effects
Cells, Cultured
Cycloheximide / pharmacology
Detergents / pharmacology
Dimethyl Sulfoxide / pharmacology
Dose-Response Relationship, Drug
Ethanol / pharmacology
Fatty Acid-Binding Proteins
Fatty Liver / etiology*,  pathology
Linoleic Acid
Linoleic Acids / pharmacology
Liver / drug effects,  metabolism,  ultrastructure
Microscopy, Electron
Microscopy, Electron, Scanning
Models, Biological*
Myelin P2 Protein / metabolism
Neoplasm Proteins*
Nerve Tissue Proteins*
Polysorbates / pharmacology*
Puromycin / pharmacology
Time Factors
Reg. No./Substance:
0/Carrier Proteins; 0/Detergents; 0/Fabp7 protein, rat; 0/Fatty Acid-Binding Proteins; 0/Linoleic Acids; 0/Myelin P2 Protein; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/Polysorbates; 2197-37-7/Linoleic Acid; 362-74-3/Bucladesine; 53-79-2/Puromycin; 64-17-5/Ethanol; 66-81-9/Cycloheximide; 67-68-5/Dimethyl Sulfoxide; 9005-66-7/polysorbate 40

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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