Document Detail

Acute rejection in heart transplant patients is associated with the presence of committed donor-specific cytotoxic lymphocytes in the graft but not in the blood.
MedLine Citation:
PMID:  1572086     Owner:  NLM     Status:  MEDLINE    
In vivo-activated, committed donor-specific cytotoxic lymphocytes (cCTL) can be propagated and expanded from endomyocardial biopsies (EMB) in IL-2-enriched medium especially during an acute rejection episode. We report here our efforts to detect these cCTL by the same technique in peripheral blood at the moment of rejection and when no rejection was diagnosed. During or just before rejection, significantly less frequent (P less than 0.01) donor reactive cCTL were found in PBL samples (two out of 20) than in the simultaneously taken EMB samples (13 out of 19). Donor B-LCL and/or third-party B-LCL were lysed by 15 PBL samples. Inhibition studies revealed that this lysis was due to LAK-like cytotoxicity. The results show that peripheral blood does not reflect intra-graft events, which is probably the reason for the irreproducible results of diagnosis of rejection by monitoring immunological parameters in the peripheral blood.
L M Vaessen; C C Baan; A J Ouwehand; N H Jutte; A H Balk; B Mochtar; F H Claas; W Weimar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  88     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-06-02     Completed Date:  1992-06-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  213-9     Citation Subset:  IM    
Department of Internal Medicine I, University Hospital Dijkzigt-Rotterdam, The Netherlands.
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MeSH Terms
Cell Line
Cytotoxicity Tests, Immunologic
Graft Rejection / immunology*
Heart Transplantation / immunology*,  pathology
Myocardium / pathology
T-Lymphocytes, Cytotoxic*

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