Document Detail


Acute pancreatitis affects non-parenchymal liver cells by a mechanism dependent on platelet-activating factor.
MedLine Citation:
PMID:  17449968     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIM: During acute experimental pancreatitis, inflammatory mediators/cytokines are released by the pancreas and enter the portal venous system, reaching the liver. We investigate some aspects of the liver cell function under conditions of acute pancreatitis and the effect of in vivo treatment with a selective platelet-activating factor (PAF) antagonist. METHODS: Cells were isolated from Wistar rats 24 h after induction of acute pancreatitis by retrograde injection of sodium taurocholate into the main pancreatic duct. The non-parenchymal cell population was separated by Percoll gradient and the adherent cell population (Kupffer cells) obtained. The cells were cultured for 24 h and supernatants assayed for nitrite by Griess reaction and for tumour necrosis factor (TNF) by bioassay in L929 cells. The microbicidal activity was evaluated by killing of Candida albicans. The PAF antagonist WEB2170 (10 mg/kg i.v.) was administered 30 min before induction of pancreatitis. RESULTS: We found that liver cells produce nitric oxide (NO) only under lipopolysaccharide stimulation and that WEB-2170 treatment reduces the NO production by liver cells in the pancreatitis group only. Cells from both groups produced TNF spontaneously, and the levels were further increased after lipopolysaccharide stimulation. WEB-2170 treatment did not affect the TNF levels. Moreover, killing of C. albicans by Kupffer cells wassignificantly increased by the PAF antagonist. CONCLUSION: These results suggest that PAF released during acute pancreatitis upregulates the NO production by non-parenchymal liver cells and inhibits Kupffer cell microbicidal activity which could explain the increased bacterial dissemination observed in acute pancreatitis.
Authors:
Lourenilson J Souza; Marina T Shio; Nilza A T Molan; Marcel C C Machado; Sonia Jancar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-18
Journal Detail:
Title:  Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]     Volume:  7     ISSN:  1424-3903     ISO Abbreviation:  Pancreatology     Publication Date:  2007  
Date Detail:
Created Date:  2007-05-14     Completed Date:  2007-07-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966936     Medline TA:  Pancreatology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  67-73     Citation Subset:  IM    
Affiliation:
Department of Surgery, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Animals
Azepines / pharmacology
Candida albicans / immunology
Kupffer Cells / immunology*
Lipopolysaccharides / pharmacology
Liver / immunology*
Male
Nitric Oxide / antagonists & inhibitors,  metabolism
Pancreatitis / chemically induced,  immunology*
Platelet Activating Factor / antagonists & inhibitors,  physiology*
Platelet Aggregation Inhibitors / pharmacology
Platelet Membrane Glycoproteins / antagonists & inhibitors
Rats
Rats, Wistar
Receptors, G-Protein-Coupled / antagonists & inhibitors
Taurocholic Acid / toxicity
Triazoles / pharmacology
Tumor Necrosis Factors / analysis,  metabolism
Chemical
Reg. No./Substance:
0/Azepines; 0/Lipopolysaccharides; 0/Platelet Activating Factor; 0/Platelet Aggregation Inhibitors; 0/Platelet Membrane Glycoproteins; 0/Receptors, G-Protein-Coupled; 0/Triazoles; 0/Tumor Necrosis Factors; 0/platelet activating factor receptor; 10102-43-9/Nitric Oxide; 114776-28-2/bepafant; 81-24-3/Taurocholic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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