Document Detail


Acute neurotoxic effects of the fungal metabolite ochratoxin-A.
MedLine Citation:
PMID:  16140385     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ochratoxin-A (OTA) is a fungal metabolite with potential toxic effects on the central nervous system that have not yet been fully characterized. OTA has complex mechanisms of action that include evocation of oxidative stress, bioenergetic compromise, inhibition of protein synthesis, production of DNA single-strand breaks and formation of OTA-DNA adducts. The time course of acute effects of OTA were investigated in the context of DNA damage, DNA repair and global oxidative stress across six brain regions. Oxidative DNA damage, as measured with the "comet assay", was significantly increased in the six brain regions at all time points up to 72 h, with peak effects noted at 24 h in midbrain (MB), CP (caudate/putamen) and HP (hippocampus). Oxidative DNA repair activity (oxyguanosine glycosylase or OGG1) was inhibited in all regions at 6 h, but recovered to control levels in cerebellum (CB) by 72 h, and showed a trend to recovery in other regions of brain. Other indices of oxidative stress were also elevated. Lipid peroxidation and superoxide dismutase (SOD) increased over time throughout the brain. In light of the known vulnerability of the nigro-striatal dopaminergic neurons to oxidative stress, levels of striatal dopamine (DA) and its metabolites were also measured. Administration of OTA (0-6 mg/kg i.p.) to mice resulted in a dose-dependent decrease in striatal DA content and turnover with an ED50 of 3.2 mg/kg. A single dose of 3.5 mg/kg decreased the intensity of tyrosine hydroxylase immunoreactivity (TH(+)) in fibers of striatum, TH(+) cells in substantia nigra (SN) and TH(+) cells of the locus ceruleus. TUNEL staining did not reveal apoptotic profiles in MB, CP or in other brain regions and did not alter DARPP32 immunoreactivity in striatum. In conclusion, OTA caused acute depletion of striatal DA on a background of globally increased oxidative stress and transient inhibition of oxidative DNA repair.
Authors:
V Sava; O Reunova; A Velasquez; R Harbison; J Sánchez-Ramos
Related Documents :
945765 - Concentrations of dopamine and norepinephrine in discrete hypothalamic nuclei during th...
6875865 - Changes in sensitivity of release modulating dopamine autoreceptors after chronic treat...
3385645 - Effects of the novel dopamine beta-hydroxylase inhibitor sk&f 102698 on catecholamines ...
2590845 - Post-mortem dopamine dynamics assessed by voltammetry and microdialysis.
24116285 - A simple behavioral paradigm to measure impulsive behavior in an animal model of attent...
2996465 - Evidence for a captopril-sensitive angiotensin converting enzyme in the hindquarter vas...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2005-09-02
Journal Detail:
Title:  Neurotoxicology     Volume:  27     ISSN:  0161-813X     ISO Abbreviation:  Neurotoxicology     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-06     Completed Date:  2007-01-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905589     Medline TA:  Neurotoxicology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  82-92     Citation Subset:  IM    
Affiliation:
University of South Florida, Department of Neurology (MDC 55), 12901 Bruce B. Downs Blvd., Tampa, FL 33612, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Brain / drug effects*,  metabolism
Caudate Nucleus / drug effects,  metabolism
Comet Assay
DNA Damage
DNA Glycosylases / metabolism
DNA Repair
Dopamine / metabolism
Dopamine and cAMP-Regulated Phosphoprotein 32 / analysis
Dose-Response Relationship, Drug
Lipid Peroxidation
Male
Mice
Mice, Inbred ICR
Mycotoxins / toxicity*
Neurons / drug effects,  metabolism
Ochratoxins / toxicity*
Oxidative Stress*
Putamen / drug effects,  metabolism
Superoxide Dismutase / metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Dopamine and cAMP-Regulated Phosphoprotein 32; 0/Mycotoxins; 0/Ochratoxins; 0/Ppp1r1b protein, mouse; 303-47-9/ochratoxin A; EC 1.15.1.1/Superoxide Dismutase; EC 3.2.2.-/DNA Glycosylases; EC 3.2.2.-/Ogg1 protein, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Permanent alterations in stress responsivity in female offspring subjected to combined maternal lead...
Next Document:  Increased manganese uptake by primary astrocyte cultures with altered iron status is mediated primar...