Document Detail

Acute molecular mechanisms responsive to feeding and meal constitution in mesenteric adipose tissue.
MedLine Citation:
PMID:  20111028     Owner:  NLM     Status:  MEDLINE    
To identify the acute effects of feeding on mesenteric fat, we have performed a transcriptomic study in the mesenteric adipose tissue after low-fat (LF) and high-fat (HF) meal ingestion. After fasting, one group of mice was killed and the others were fed ad libitum with HF or LF meal, and killed 3 h after the ingestion. Serial analysis of gene expression (SAGE) was performed, generating approximately 150,000 tags/sample. The results were confirmed using quantitative real-time PCR (qRT-PCR). Transcripts involved in lipid biosynthesis were upregulated only by LF meal, whereas intracellular lipid catabolism was repressed by feeding. Apoptotic genes were downregulated, whereas antiapoptosis and proteolysis were upregulated by feeding. The expression levels of genes coding for adiponectin and ribosomal proteins were decreased by HF meal, as well as transcripts involved in mRNA processing, cytoskeleton, and extracellular matrix. Several other fat-responsive genes were identified, including diverse uncharacterized transcripts. These results revealed that mesenteric adipose tissue transcriptome was responsive to food intake and was affected differently according to meal constitution. The identification of uncharacterized transcripts regulated by LF and HF meals is a first step toward further understanding the early mechanisms of diet-induced obesity as well as discovering new therapeutic targets for obesity-related diseases.
Carl Bolduc; Mayumi Yoshioka; Jonny St-Amand
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-20
Journal Detail:
Title:  Obesity (Silver Spring, Md.)     Volume:  18     ISSN:  1930-739X     ISO Abbreviation:  Obesity (Silver Spring)     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-29     Completed Date:  2010-02-22     Revised Date:  2012-08-13    
Medline Journal Info:
Nlm Unique ID:  101264860     Medline TA:  Obesity (Silver Spring)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  410-3     Citation Subset:  IM    
Functional Genomics Laboratory, Department of Anatomy and Physiology, Molecular Endocrinology and Oncology Research Center, Laval University Medical Center, Laval University, Québec, Quebec, Canada.
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MeSH Terms
Abdominal Fat / metabolism*
Apoptosis / genetics
Cytoskeleton / genetics
Diet, Fat-Restricted
Dietary Fats / metabolism*
Extracellular Matrix / genetics
Gene Expression Profiling / methods
Gene Expression Regulation
Lipid Metabolism / genetics
Mice, Inbred C57BL
Obesity / genetics,  metabolism*
Polymerase Chain Reaction
RNA, Messenger / metabolism
Grant Support
//Canadian Institutes of Health Research
Reg. No./Substance:
0/Dietary Fats; 0/RNA, Messenger

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