Document Detail


Acute lymphoblastic leukemia with azurophilic granules that contain ultrastructural myeloperoxidase activity.
MedLine Citation:
PMID:  1707229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present a case of acute lymphoblastic leukemia (ALL) in which the leukemic cells had cytoplasmic azurophilic granules. Surface marker studies revealed that the leukemic cells expressed CD10 (CALLA), CD19, CD20, and HLA-DR antigens. Cytochemical studies by light microscopy revealed that the blasts were negative for myeloperoxidase, PAS staining, and double esterase staining, supporting the diagnosis of ALL. However, an ultrastructural study demonstrated that some of the cytoplasmic granules were myeloperoxidase (MPO) positive. Our findings suggested that leukemic transformation in this case may have taken place at a stage ontogenetically close to the pluripotent stem cell. Furthermore, the present case indicates the existence of a new form of ALL is characterized by MPO-positive granules detectable by ultracytochemistry and lymphoid-associated surface markers.
Authors:
T Tauchi; K Ohyashiki; J H Ohyashiki; M Saito; S Nakazawa; Y Enomoto; Y Hosoda; K Toyama
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  American journal of hematology     Volume:  36     ISSN:  0361-8609     ISO Abbreviation:  Am. J. Hematol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-05-06     Completed Date:  1991-05-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7610369     Medline TA:  Am J Hematol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  288-90     Citation Subset:  IM    
Affiliation:
First Department of Internal Medicine, Tokyo Medical College, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD19
Antigens, CD20
Antigens, Differentiation / metabolism
Antigens, Differentiation, B-Lymphocyte / metabolism
Antigens, Neoplasm / metabolism
Azure Stains / diagnostic use
Cytoplasmic Granules / enzymology*,  ultrastructure
Female
Genotype
HLA-DR Antigens / metabolism
Histocytochemistry / methods
Humans
Lymphocyte Activation
Microscopy, Electron / methods
Middle Aged
Neprilysin
Peroxidase / metabolism*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics,  metabolism,  pathology*
Chemical
Reg. No./Substance:
0/Antigens, CD19; 0/Antigens, CD20; 0/Antigens, Differentiation; 0/Antigens, Differentiation, B-Lymphocyte; 0/Antigens, Neoplasm; 0/Azure Stains; 0/HLA-DR Antigens; EC 1.11.1.7/Peroxidase; EC 3.4.24.11/Neprilysin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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