| Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules. | |
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MedLine Citation:
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PMID: 7513171 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Serial biopsies from 41 consecutive renal allotransplanted patients were evaluated in order to obtain pretransplant data as well as information on well-functioning and acutely rejecting grafts. Each patient served as his own control. Thirty-five patients were followed according to the schedule which included biopsy prior to transplantation, shortly after opening of reanastomosis, at least once postoperatively (days 7-10), and furthermore whenever clinically indicated. The morphological evaluation was in each case combined with immunofluorescence (to detect immunoglobulins and complement fractions) and immunohistochemistry with a wide panel of monoclonal antibodies for T cells (CD2, CD3, CD4, CD8, gamma delta), B cells (CD20, CD22), macrophages (CD68, MAC387) NK cells (leu-7, CD16), activation markers (IL-2-R, Ki-67, transferrin-R), MHC antigens (HLA-ABC, HLA-DR), adhesion molecules (ICAM-1, VCAM-1, ELAM-1, PADGEM, VLA-4, LFA-1 alpha/beta), and growth factors (EGF, TGF-alpha, EGF-R). When 132 biopsies and 10 failed allografts were examined, no specific morphological or immunohistological parameter predictive of rejection or graft outcome could be found. Morphology in follow-up biopsies from non-rejecting and rejecting patients revealed a continuum of inflammatory changes, and several non-rejecting cases demonstrated cellular inflammatory infiltrates which could not be discriminated from those seen in acute rejection. Of the patients 44% had acute rejection accompanied by increased infiltration of T cells and macrophages showing enhanced IL-2-R expression, increased tubular and endothelial staining for MHC class II, ICAM-1, and VCAM-1, and strong leukocytic expression of VLA-4 and LFA-1 alpha/beta. |
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Authors:
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C B Andersen; S D Ladefoged; S Larsen |
Publication Detail:
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Type: Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica Volume: 102 ISSN: 0903-4641 ISO Abbreviation: APMIS Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-06-01 Completed Date: 1994-06-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8803400 Medline TA: APMIS Country: DENMARK |
Other Details:
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Languages: eng Pagination: 23-37 Citation Subset: IM |
Affiliation:
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Department of Pathology, Herlev Hospital, University of Copenhagen, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Antigens, CD / analysis, immunology Antigens, CD2 Antigens, CD20 Antigens, CD22 Antigens, CD3 / analysis, immunology Antigens, CD4 / analysis, immunology Antigens, CD8 / analysis, immunology Antigens, Differentiation, B-Lymphocyte / analysis, immunology Antigens, Differentiation, Myelomonocytic / analysis, immunology Antigens, Differentiation, T-Lymphocyte / analysis, immunology B-Lymphocytes / immunology, pathology, ultrastructure Cell Adhesion Molecules / analysis, immunology Cell Movement / physiology Child Complement System Proteins / analysis, immunology Double-Blind Method Epidermal Growth Factor / analysis Fluorescent Antibody Technique Follow-Up Studies Graft Rejection / epidemiology, immunology, pathology HLA Antigens / analysis, immunology Humans Immunoglobulins / analysis, immunology Immunohistochemistry Intercellular Adhesion Molecule-1 Kidney / chemistry, immunology, pathology Kidney Transplantation / immunology*, pathology Killer Cells, Natural / immunology, pathology Lectins* Macrophages / immunology, pathology Middle Aged Receptors, IgG / analysis, immunology Receptors, Immunologic / analysis, immunology Receptors, Interleukin-2 / analysis T-Lymphocytes / immunology, pathology, ultrastructure Time Factors Transplantation, Homologous |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, CD2; 0/Antigens, CD20; 0/Antigens, CD22; 0/Antigens, CD3; 0/Antigens, CD4; 0/Antigens, CD8; 0/Antigens, Differentiation, B-Lymphocyte; 0/Antigens, Differentiation, Myelomonocytic; 0/Antigens, Differentiation, T-Lymphocyte; 0/CD22 protein, human; 0/CD68 antigen, human; 0/Cell Adhesion Molecules; 0/HLA Antigens; 0/Immunoglobulins; 0/Lectins; 0/Receptors, IgG; 0/Receptors, Immunologic; 0/Receptors, Interleukin-2; 126547-89-5/Intercellular Adhesion Molecule-1; 62229-50-9/Epidermal Growth Factor; 9007-36-7/Complement System Proteins |
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