| Acute inhibition of carboxypeptidase E expression in AtT-20 cells does not affect regulated secretion of ACTH. | |
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MedLine Citation:
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PMID: 20655338 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Carboxypeptidase E (CPE) is an exopeptidase that removes C-terminal basic amino acids from a variety of bioactive peptides. In addition to this role, data obtained in recent years has supported a potential function for CPE as a sorting receptor, helping direct peptides destined for regulated secretion from the trans-Golgi to granules in preparation for release. This possible sorting function was assessed using mouse AtT-20 cells, a well-established corticotroph cell line that synthesizes and releases POMC/ACTH in regulated fashion. Cells that were treated with siRNA to Cpe effectively suppressed CPE expression. ACTH was released in a regulated fashion from CPE-depleted cells in response to two secretagogues, 8-bromo-cyclic AMP and corticotrophin-releasing hormone. POMC/ACTH content of CPE-depleted cells was higher than that of control cells, but both released a similar percentage of ACTH content in response to secretagogue addition. Cells depleted of CPE generally secreted more high-molecular weight forms of POMC/ACTH under basal conditions than control cells; however, the CPE-depleted cells responded to a secretagogue by releasing newly synthesized ACTH 1-39 in a manner similar to controls. These results, whereby RNAi was used to acutely suppress CPE, do not support a role for this protein as necessary for or central to sorting of POMC/ACTH to the regulated secretory pathway in AtT-20 cells. |
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Authors:
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Robert J Kemppainen; Ellen N Behrend |
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Publication Detail:
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Type: Journal Article Date: 2010-07-22 |
Journal Detail:
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Title: Regulatory peptides Volume: 165 ISSN: 1873-1686 ISO Abbreviation: Regul. Pept. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100479 Medline TA: Regul Pept Country: Netherlands |
Other Details:
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Languages: eng Pagination: 174-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Departments of Anatomy, Physiology, and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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