Document Detail

Acute hepatic response to aflatoxin B1 in rats fed a methyl-deficient, amino acid-defined diet.
MedLine Citation:
PMID:  8098659     Owner:  NLM     Status:  MEDLINE    
In order to evaluate the relative contribution of aflatoxin B1 (AFB1)-induced toxicity towards a methyl-deficient diet influenced AFB1 carcinogenesis, a no-observed-effect-level (NOEL) for AFB1, with reference to liver damage, was determined in rats fed a nutritionally complete amino acid-defined basal (CMS) diet or a choline-methionine-deficient (CMD) diet. After 3 weeks of dietary treatment, male Fischer 344 rats received a single, oral dose of AFB1 in the range of 100-600 pg/kg body weight. At 24, 48 and 72 h after AFB1 treatment, six serum biochemical parameters were analysed in parallel with histological examination of liver sections. In rats fed the CMS diet and receiving 250-600 micrograms/kg AFB1, serum levels of glutamyl oxalo-transaminase (SGOT), glutamyl pyruvic transaminase (SGPT), alkaline phosphatase (ALP) and total bilirubin increased, glucose levels decreased and gamma glutamyl transpeptidase (GGT) levels remained unchanged over the 72-h period following mycotoxin treatment. However, at 100 micrograms/kg AFB1, these serum parameters remained at control levels. Pathological examination of liver sections indicated no significant lesions at 100 micrograms/kg AFB1 confirming this as the non-necrogenic dose or NOEL in CMS diet group rats. In contrast, in CMD diet fed rats, serum or pathology data showed no obvious time- or dose-response to mycotoxin treatment, extensive hepatic lipidosis in response to dietary treatment being the only predominant lesion in this diet group. The milder response of CMD rat livers to a single dose of AFB1 suggest a possible reduction in the susceptibility of these livers to AFB1 toxicity.
R Mehta; J S Campbell; G W Laver; R Stapley; R Mueller
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer letters     Volume:  69     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-06-23     Completed Date:  1993-06-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  93-106     Citation Subset:  IM    
Toxicology Research Division, Health and Welfare Canada, Ottawa, Ontario.
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MeSH Terms
Aflatoxin B1 / administration & dosage*
Alanine Transaminase / blood
Aspartate Aminotransferases / blood
Bilirubin / blood
Blood Glucose / metabolism
Body Weight / drug effects
Choline / metabolism
Dose-Response Relationship, Drug
Feeding Behavior
Liver / drug effects*,  pathology
Methionine / metabolism
Rats, Inbred F344
gamma-Glutamyltransferase / blood
Reg. No./Substance:
0/Blood Glucose; 1162-65-8/Aflatoxin B1; 62-49-7/Choline; 63-68-3/Methionine; 635-65-4/Bilirubin; EC; EC Aminotransferases; EC Transaminase
Erratum In:
Cancer Lett 1993 Aug 16;72(1-2):135

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