Document Detail


Acute heat treatment improves insulin-stimulated glucose uptake in aged skeletal muscle.
MedLine Citation:
PMID:  21148343     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aging is associated with insulin resistance and decreased insulin-stimulated glucose uptake into skeletal muscle. Although the mechanisms underlying age-related insulin resistance are not clearly defined, impaired defense against inflammation and tissue oxidative stress are likely causes. Heat shock proteins (HSPs) have been shown to protect tissue from oxidative stress and inhibit the activation of stress kinases such as JNK, known to interfere with the insulin signaling pathway. While the induction of HSPs via chronic heat treatment has been shown to protect skeletal muscle from obesity-related insulin resistance, the ability of heat treatment to improve insulin action in aged skeletal muscle is not known. In the present study, one bout of in vivo heat treatment applied to 24-mo-old Fischer 344 rats improved insulin-stimulated glucose uptake after 24 h in slow-twitch soleus muscles. In vitro heat treatment applied to young (3-mo-old) and aged (24-mo-old) soleus muscles increased expression of HSP72 and inhibited anisomycin-induced activation of JNK. In contrast, heat treatment had no effect on p38 MAPK, a MAPK strongly activated with anisomycin. Prior inhibition of HSP72 transcription with the pharmacological inhibitor KNK437 eliminated the ability of heat treatment to blunt JNK activation. This suggests that the ability of heat treatment to inhibit JNK activation in skeletal muscle is dependent on increased HSP72 expression. In conclusion, an acute bout of heat treatment can increase insulin-stimulated glucose uptake in aged skeletal muscle, with the underlying mechanism likely to be HSP72-mediated JNK inhibition.
Authors:
Anisha A Gupte; Gregory L Bomhoff; Chad D Touchberry; Paige C Geiger
Related Documents :
21414423 - Chronic assessment of diaphragm muscle emg activity across motor behaviors.
16199043 - Reproductive potential of echinococcus multilocularis in experimentally infected foxes,...
7381773 - Force staircase kinetics in mammalian cardiac muscle: modulation by muscle length.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-12-09
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  110     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-09     Completed Date:  2011-06-07     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  451-7     Citation Subset:  IM    
Affiliation:
Dept. of Molecular and Integrative Physiology, Univ. of Kansas Medical Center, MS 3043, 3901 Rainbow Blvd., Kansas City, KS 66160, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aging / metabolism*
Animals
Glucose / pharmacokinetics*
Hyperthermia, Induced / methods*
Insulin / metabolism*,  pharmacology
Insulin Resistance / physiology*
Muscle, Skeletal / drug effects,  metabolism*
Rats
Rats, Inbred F344
Grant Support
ID/Acronym/Agency:
AG-031575/AG/NIA NIH HHS; HD-002528/HD/NICHD NIH HHS; P20 RR-016475/RR/NCRR NIH HHS; R01 AG031575-02/AG/NIA NIH HHS; R01 AG031575-03/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Insulin; 50-99-7/Glucose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Left ventricular epicardial admittance measurement for detection of acute LV dilation.
Next Document:  Decreased clearance of CNS beta-amyloid in Alzheimer's disease.