Document Detail

Acute food deprivation and chronic food restriction differentially affect hypothalamic NPY mRNA expression.
MedLine Citation:
PMID:  12842868     Owner:  NLM     Status:  MEDLINE    
Although acute food deprivation and chronic food restriction both result in body weight loss, they produce different metabolic states. To evaluate how these two treatments affect hypothalamic peptide systems involved in energy homeostasis, we compared patterns of hypothalamic neuropeptide Y (NPY), agouti-related protein (AgRP), proopiomelanocotin (POMC), and leptin receptor gene expression in acutely food-deprived and chronically food-restricted rats. Both acute food deprivation and chronic food restriction reduced body weight and circulating leptin levels and resulted in increased arcuate NPY and decreased arcuate POMC gene expression. Arcuate AgRP mRNA levels were only elevated in acutely deprived rats. NPY gene expression was increased in the compact subregion of the dorsomedial hypothalamus (DMH) in response to chronic food restriction, but not in response to acute food deprivation. Leptin receptor expression was not affected by either treatment. Double in situ hybridization histochemistry revealed that, in contrast to the situation in the arcuate nucleus, NPY and leptin receptor mRNA-expressing neurons were not colocalized in the DMH. Together, these data suggest that arcuate and DMH NPY gene expression are differentially regulated. DMH NPY-expressing neurons do not appear to be under the direct control of leptin signaling.
Sheng Bi; Benjamin M Robinson; Timothy H Moran
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2003-07-03
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  285     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-14     Completed Date:  2003-11-21     Revised Date:  2011-08-03    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R1030-6     Citation Subset:  IM    
Dept. of Psychiatry and Behavioral Sciences, Johns Hopkins Univ. School of Medicine, 720 Rutland Ave., Ross 618, Baltimore, MD 21205, USA.
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MeSH Terms
Agouti-Related Protein
Blood Glucose / metabolism
Body Weight / physiology
Dorsomedial Hypothalamic Nucleus / physiology*
Energy Intake / physiology*
Food Deprivation / physiology*
Gene Expression / physiology
Insulin / blood
Intercellular Signaling Peptides and Proteins
Leptin / blood
Neuropeptide Y / genetics*
Pro-Opiomelanocortin / genetics
Proteins / genetics
RNA, Messenger / analysis
Rats, Sprague-Dawley
Receptors, Cell Surface / genetics
Receptors, Leptin
Grant Support
Reg. No./Substance:
0/AGRP protein, rat; 0/Agouti-Related Protein; 0/Blood Glucose; 0/Intercellular Signaling Peptides and Proteins; 0/Leptin; 0/Neuropeptide Y; 0/Proteins; 0/RNA, Messenger; 0/Receptors, Cell Surface; 0/Receptors, Leptin; 11061-68-0/Insulin; 66796-54-1/Pro-Opiomelanocortin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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