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Acute exercise induce endothelial nitric oxide synthase phosphorylation via Akt and AMP-activated protein kinase in aorta of rats: Role of reactive oxygen species.
MedLine Citation:
PMID:  22989604     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Acute exercise increases reactive oxygen species (ROS) levels, including hydrogen peroxide (H(2)O(2)). H(2)O(2) promotes endothelial nitric oxide synthase (eNOS) activation and phosphorylation in endothelial cells. With this in mind, the present study was designed to evaluate ex vivo eNOS phosphorylation in rat aortas incubated with H(2)O(2) and to test this hypothesis in vivo in the aortas of rats submitted to acute exercise. METHODS: For ex vivo studies, six groups of aortic tissue were formed: control, H(2)O(2), N-acetylcysteine (NAC), LY294002, compound C, and LY294002 plus compound C. While incubation with H(2)O(2) increased Akt, AMPK and eNOS phosphorylation, pre-incubation with NAC strongly reduced the phosphorylation of these enzymes. For in vivo studies, male Wistar rats were divided into four groups: control, cont+NAC, exercise, and exer+NAC. After a 3h swimming session, animals were decapitated and aortas were excised for biochemical and immunoblotting analysis. RESULTS: Acute exercise increased superoxide levels and dichlorofluorescein (DCF) concentrations, and this increase was related to phosphorylation of Akt, AMPK and eNOS. On the other hand, use of NAC reduced superoxide levels and DCF concentration. Reduced superoxide levels and DCF in the exer+NAC group were associated with decreased Akt, AMPK and eNOS phosphorylation. These results appear to be connected with vascular function because VASP phosphorylation increased in acute exercise and decreased in exer+NAC. CONCLUSION: Our results indicate that ROS induced by acute exercise play the important role of activating eNOS, a process apparently mediated by Akt and AMPK.
Authors:
Viviane A Barbosa; Thais F Luciano; Scherolin O Marques; Marcelo F Vitto; Daniela R Souza; Luciano A Silva; João Paulo A Santos; José C Moreira; Felipe Dal-Pizzol; Fabio S Lira; Ricardo A Pinho; Claudio T De Souza
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-15
Journal Detail:
Title:  International journal of cardiology     Volume:  -     ISSN:  1874-1754     ISO Abbreviation:  Int. J. Cardiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8200291     Medline TA:  Int J Cardiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Laboratory of Exercise Biochemistry and Physiology, Postgraduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
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