Document Detail


Acute exercise alters Galphai2 protein expressions through the ubiquitin-proteasome proteolysis pathway in rat adipocytes.
MedLine Citation:
PMID:  15381114     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of acute exercise on the protein expressions of heterotrimeric G protein alpha subunits were examined in rat adipocytes. Galphai2 protein expression was significantly reduced 0 and 3h after exercise but increased 24h after exercise, without alterations in Galphai2 mRNA expressions. The protein expressions of other alpha subunits, Galphas, Galphai1, and Galphai3, were not influenced. Both the 26S proteasome activity and polyubiquitination of Galphai2 protein were significantly increased 0 and 3h after exercise. Whereas, proteasome activity was decreased, and the polyubiquitination of Galphai2 protein was returned to the control level 24h after exercise. The reductions in Galphai2 protein expressions 0 and 3h after exercise were completely prevented by the injection either of a proteasome inhibitor or of a beta-adrenergic receptor blocker prior to exercise. Thus, acute exercise altered the expression of Galphai2 protein via mechanisms which involve the coupling of beta-adrenergic receptors to an agonist with subsequent ubiquitin-proteasome-dependent proteolysis.
Authors:
Junetsu Ogasawara; Takuya Sakurai; Nazibur Rahman; Takako Kizaki; Yoshiaki Hitomi; Hideki Ohno; Tetsuya Izawa
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  323     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-21     Completed Date:  2004-12-09     Revised Date:  2012-07-13    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1109-15     Citation Subset:  IM    
Affiliation:
Department of Kinesiology, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / analogs & derivatives*,  pharmacology
Adaptation, Physiological / drug effects,  physiology
Adipocytes / drug effects,  physiology*
Animals
Cells, Cultured
Enzyme Activation
GTP-Binding Protein alpha Subunit, Gi2
GTP-Binding Protein alpha Subunits, Gi-Go / metabolism*
Gene Expression Regulation / physiology
Male
Multienzyme Complexes
Peptide Hydrolases / metabolism
Physical Conditioning, Animal / methods*
Physical Endurance / physiology*
Propranolol / pharmacology
Proteasome Endopeptidase Complex / metabolism*
Proto-Oncogene Proteins / metabolism*
Rats
Rats, Wistar
Signal Transduction / physiology*
Ubiquitin / metabolism*
Chemical
Reg. No./Substance:
0/Multienzyme Complexes; 0/Proto-Oncogene Proteins; 0/Ubiquitin; 133343-34-7/lactacystin; 525-66-6/Propranolol; 616-91-1/Acetylcysteine; EC 3.4.-/Peptide Hydrolases; EC 3.4.25.1/Proteasome Endopeptidase Complex; EC 3.6.5.1/GTP-Binding Protein alpha Subunit, Gi2; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, Gi-Go; EC 3.6.5.1/Gnai2 protein, rat

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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