Document Detail


Acute exercise activates myocardial nuclear factor kappa B.
MedLine Citation:
PMID:  20694538     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The myocardial stress response to exercise is dependent on exercise intensity and thus understanding the molecular responses between various exercise intensity levels might aid in exercise prescription. Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor that mediates a variety of cellular processes including inflammation, immune responses, apoptosis and cell growth/development. NF-κB can be comprised of homo- and/or heterodimers formed from five distinct proteins: p50 (NF-κB1), p52 (NF-κB2), RelA (p65), c-Rel, and RelB. NF-κB is located in the cytoplasm and kept inactive by inhibitory proteins but following the exposure to a myriad of stimuli, an activated NF-κB dimer translocates to the nucleus and exerts transcriptional effects on upwards of 150 genes. To examine the activation of NF-κB in the myocardium following exercise, male Sprague-Dawley rats (n = 24) were exercised by treadmill running at 20 m/min for 30 min or 30 m/min for 20 min. At 0, 2, or 24 h following exercise, animals were anesthetized, hearts excised and immediately frozen in liquid nitrogen. Portions of hearts were homogenized, protein concentrations determined and extracts assayed for NF-κB activation (DNA binding activity) using electrophoretic mobility shift assays (EMSA). Visual examination of EMSA autoradiographs revealed an enhanced NF-κB activation in the hearts from exercised animals when compared with non-running controls. Subsequent supershift analyses using antibodies specific for NF-κB subunits showed the higher intensity exercise was associated with p65 (RelA) in the activated NF-κB complex while the NF-κB complex in hearts from animals exercised at the lower intensity was comprised primarily of p50. These data suggest exercise is capable of activating myocardial NF-κB and that a threshold for the activation of specific NF-κB subunits may exist.
Authors:
Marko Balan; Marius Locke
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Publication Detail:
Type:  Journal Article     Date:  2010-08-06
Journal Detail:
Title:  Cell stress & chaperones     Volume:  16     ISSN:  1466-1268     ISO Abbreviation:  Cell Stress Chaperones     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-02     Completed Date:  2011-03-16     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9610925     Medline TA:  Cell Stress Chaperones     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  105-11     Citation Subset:  IM    
Affiliation:
Faculty of Physical Education and Health, University of Toronto, 55 Harbord Street, Toronto, ON, M5S 2W6, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dimerization
Electrophoretic Mobility Shift Assay
Male
Myocardium / metabolism*
NF-kappa B / metabolism*
NF-kappa B p50 Subunit / metabolism
NF-kappa B p52 Subunit / metabolism
Physical Conditioning, Animal
Rats
Rats, Sprague-Dawley
Temperature
Time Factors
Transcription Factor AP-1 / metabolism
Transcription Factor RelA / metabolism
Transcription Factor RelB / metabolism
Chemical
Reg. No./Substance:
0/NF-kappa B; 0/NF-kappa B p50 Subunit; 0/NF-kappa B p52 Subunit; 0/Transcription Factor AP-1; 0/Transcription Factor RelA; 147337-75-5/Transcription Factor RelB
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