Document Detail

Acute ethanol exposure attenuates expression of junD in human neuroblastoma cells.
MedLine Citation:
PMID:  10098722     Owner:  NLM     Status:  MEDLINE    
This study describes the effects of acute ethanol exposure on the mRNA levels of c-jun,junB and junD in the human neuroblastoma SH-SY5Y cell line. An acute exposure to 100 mM ethanol did not influence the basal and phorbol ester-induced expression of c-jun and junB, whereas the basal mRNA level of junD was attenuated by 30%. This effect was dose- and time-dependent with maximal inhibition being detected 2 h after 100 mM ethanol treatment and the mRNA levels gradually returned towards normal afterwards. Ethanol also inhibited phorbol ester-induced expression of junD. The fact that ethanol did not influence degradation of the junD mRNA suggests that acute ethanol suppresses the transcription of the gene. These results indicate that acute ethanol exerts different effects on expression of Jun transcription factors, suggesting that as compared to c-jun and junB, the junD gene may be more sensitive to acute ethanol treatment in neuronal cells.
W Q Ding; U Fried; C Larsson; C Alling
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemistry international     Volume:  33     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-05-06     Completed Date:  1999-05-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  525-30     Citation Subset:  IM    
Department of Medical Neurochemistry, Institute of Laboratory Medicine, Lund University Hospital, Sweden.
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MeSH Terms
Dactinomycin / pharmacology
Ethanol / pharmacology*
Gene Expression Regulation / drug effects*
Proto-Oncogene Proteins c-jun / genetics*
RNA, Messenger / genetics,  metabolism
Signal Transduction
Tetradecanoylphorbol Acetate / pharmacology
Tumor Cells, Cultured
Reg. No./Substance:
0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 16561-29-8/Tetradecanoylphorbol Acetate; 50-76-0/Dactinomycin; 64-17-5/Ethanol

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