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Acute effects of microcystins on the transcription of 14 glutathione S-transferase isoforms in Wistar rat.
MedLine Citation:
PMID:  19790251     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The glutathione S-transferases (GST) play important roles in the detoxification of microcystins (MCs). For better understanding of the responses of GST isforms to MCs exposure, informations about the effects of MCs on GSTs are necessary. In this experiment, we cloned the full length cDNA of 14 GST isoforms (GST alpha, kappa, mu, omega, pi, theta, zeta, and microsomal GST) from Wistar rat. The mRNA abundance of each rat GST isoform in the liver, kidney, and testis was analyzed by real time quantitative PCR. Multiple GST isoforms were constitutively expressed in all examined organs, but some isoforms were expressed at higher level in one organ than in others. The relative changes of the mRNA abundance in the liver, kidney, and testis of Wiatar rat i.v. injected with crude MCs extract at dose of 1LD(50) were also analyzed. Generally, the expression of most GSTs in the liver and testis was suppressed while that in kidney was induced after being injected with MCs. It is suggested that the transcription of GST isoforms varied in different ways within an organ and between organs of Wistar rat exposed to MCs. © 2009 Wiley Periodicals, Inc. Environ Toxicol 26: 187-194, 2011.
Authors:
Guangyu Li; Ping Xie; Huiying Li; Le Hao; Qian Xiong; Tong Qiu; Ying Liu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Environmental toxicology     Volume:  26     ISSN:  1522-7278     ISO Abbreviation:  Environ. Toxicol.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100885357     Medline TA:  Environ Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  187-94     Citation Subset:  IM    
Copyright Information:
Copyright © 2009 Wiley Periodicals, Inc.
Affiliation:
Donghu Experimental Station of Lake Ecosystems, State Key Laboratory for Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, The Chinese Academy of Sciences, Donghu South Road 7, Wuhan 430072, People's Republic of China.
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