| Acute effects of Helicobacter pylori extracts on gastric mucosal blood flow in the mouse. | |
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MedLine Citation:
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PMID: 19132773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the mechanisms underlying the reduction in gastric blood flow induced by a luminal water extract of Helicobacter pylori (HPE). METHODS: The stomachs of isoflurane-anesthetized mice were exteriorized, and the mucosal surface exposed. Blood flow was measured with the laser-Doppler technique, and systemic arterial blood pressure monitored. C57BL/6 mice were exposed to water extract produced from H pylori strain 88-23. To investigate the role of a nerve- or iNOS-mediated pathway, we used intraluminal lidocaine and iNOS-/- mice. Blood flow response to the endogenous nitric oxide synthase inhibitor asymmetric dimethyl arginine (ADMA) was also assessed. RESULTS: In wild-type mice, HPE decreased mucosal blood flow by approximately 30%. This reduction was abolished in iNOS-deficient mice, and by pre-treatment with lidocaine. Luminally applied ADMA resulted in reduction in blood flow similar to that observed in wild-type mice exposed to HPE. CONCLUSION: A H pylori water extract reduces gastric mucosal blood flow acutely through iNOS- and nerve-mediated pathways. |
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Authors:
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Johanna Henriksnäs; Christer Atuma; Mia Phillipson; Stellan Sandler; Lars Engstrand; Lena Holm |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 15 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-09 Completed Date: 2009-04-21 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 219-25 Citation Subset: IM |
Affiliation:
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Department of Medical Cell Biology, Uppsala University, PO Box 571, SE-751 23 Uppsala, Sweden. johanna.henriksnas@mcb.uu.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine / analogs & derivatives, pharmacology Blood Flow Velocity / drug effects Gastric Mucosa / blood supply*, drug effects, innervation, microbiology* Helicobacter pylori / chemistry, pathogenicity* Lidocaine / pharmacology Male Mice Mice, Inbred C57BL Mice, Knockout Nitric Oxide Synthase Type II / deficiency, genetics, physiology Signal Transduction / drug effects Water |
| Chemical | |
Reg. No./Substance:
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137-58-6/Lidocaine; 30315-93-6/N,N-dimethylarginine; 74-79-3/Arginine; 7732-18-5/Water; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nos2 protein, mouse |
| Comments/Corrections | |
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