Document Detail


Acute effects of Helicobacter pylori extracts on gastric mucosal blood flow in the mouse.
MedLine Citation:
PMID:  19132773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the mechanisms underlying the reduction in gastric blood flow induced by a luminal water extract of Helicobacter pylori (HPE). METHODS: The stomachs of isoflurane-anesthetized mice were exteriorized, and the mucosal surface exposed. Blood flow was measured with the laser-Doppler technique, and systemic arterial blood pressure monitored. C57BL/6 mice were exposed to water extract produced from H pylori strain 88-23. To investigate the role of a nerve- or iNOS-mediated pathway, we used intraluminal lidocaine and iNOS-/- mice. Blood flow response to the endogenous nitric oxide synthase inhibitor asymmetric dimethyl arginine (ADMA) was also assessed. RESULTS: In wild-type mice, HPE decreased mucosal blood flow by approximately 30%. This reduction was abolished in iNOS-deficient mice, and by pre-treatment with lidocaine. Luminally applied ADMA resulted in reduction in blood flow similar to that observed in wild-type mice exposed to HPE. CONCLUSION: A H pylori water extract reduces gastric mucosal blood flow acutely through iNOS- and nerve-mediated pathways.
Authors:
Johanna Henriksnäs; Christer Atuma; Mia Phillipson; Stellan Sandler; Lars Engstrand; Lena Holm
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  15     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-09     Completed Date:  2009-04-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  219-25     Citation Subset:  IM    
Affiliation:
Department of Medical Cell Biology, Uppsala University, PO Box 571, SE-751 23 Uppsala, Sweden. johanna.henriksnas@mcb.uu.se
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MeSH Terms
Descriptor/Qualifier:
Animals
Arginine / analogs & derivatives,  pharmacology
Blood Flow Velocity / drug effects
Gastric Mucosa / blood supply*,  drug effects,  innervation,  microbiology*
Helicobacter pylori / chemistry,  pathogenicity*
Lidocaine / pharmacology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Nitric Oxide Synthase Type II / deficiency,  genetics,  physiology
Signal Transduction / drug effects
Water
Chemical
Reg. No./Substance:
137-58-6/Lidocaine; 30315-93-6/N,N-dimethylarginine; 74-79-3/Arginine; 7732-18-5/Water; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nos2 protein, mouse
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