Document Detail


Acute edematous and necrotic pancreatitis in a porcine model.
MedLine Citation:
PMID:  18609130     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: It is unclear why pancreatitis progresses either to mild edematous disease or to severe necrotic disease. The aim of the study was to shed some light on this topic by investigating differences during the early stages of necrotic and edematous pancreatitis. MATERIAL AND METHODS: Piglets were randomized into two groups. Necrotic pancreatitis was induced with retrograde injection of 20% taurocholic acid (1 ml/kg), and edematous pancreatitis was induced with 0.9% NaCl (1 ml/kg). Central hemodynamics was measured, and pancreatic microcirculation was directly examined by intravital microscopy. Vascular permeability to proteins and albumin was measured by microdialysis. Apoptosis and claudins 2, 3, 4, 5, and 7 were analyzed from pancreatic tissue samples. Blood samples were taken for analysis of blood cell counts, blood gases, lipase, and amylase. RESULTS: Hemodynamic changes were similar in both groups, whereas microcirculatory impairment was more pronounced in necrotic pancreatitis. Necrosis was associated only with necrotic pancreatitis. Apoptosis increased only in edematous pancreatitis. The number of blood neutrophils and monocytes increased and lymphocyte and platelet counts decreased in both groups. Necrotic pancreatitis was associated with increased permeability to albumin and proteins. Expression of claudins 3, 4, 5, and 7 was not changed during pacreatitis, but in acinar cells, membranous expression of claudin-2 increased in both groups. CONCLUSIONS: The results show that acute edematous pancreatitis is characterized by induction of apoptosis, whereas full-blown pancreatitis is characterized by necrosis. Impaired vascular permeability to albumin and protein is related to the early phase of necrotic pancreatitis. Claudin-2 increases during acute necrotic and edematous pancreatitis and may be related to impaired permeability.
Authors:
Sanna Meriläinen; Jyrki Mäkelä; Vesa Anttila; Vesa Koivukangas; Hanna Kaakinen; Eija Niemelä; Pasi Ohtonen; Juha Risteli; Tuomo Karttunen; Ylermi Soini; Tatu Juvonen
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of gastroenterology     Volume:  43     ISSN:  1502-7708     ISO Abbreviation:  Scand. J. Gastroenterol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-09-19     Completed Date:  2009-02-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0060105     Medline TA:  Scand J Gastroenterol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  1259-68     Citation Subset:  IM    
Affiliation:
Department of Surgery, Oulu University Hospital, University of Oulu, Oulu, Finland. memerila@paju.oulu.fi
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Capillary Permeability
Disease Models, Animal
Edema / etiology*
Microcirculation
Pancreas / blood supply*
Pancreatitis, Acute Necrotizing / chemically induced,  pathology*,  physiopathology*
Severity of Illness Index
Swine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Prophylactic antibiotic treatment in acute necrotizing pancreatitis: results from a meta-analysis.
Next Document:  Reconstitution of stratified murine and human oesophageal epithelia in an in vivo transplant culture...