| Acute circuit-resistance exercise increases expression of lymphocyte agouti-related protein in young women. | |
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MedLine Citation:
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PMID: 20404050 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exercise-induced leukocytosis and lymphocytosis is accompanied by up-regulation and down-regulation of hundreds of genes in white blood cells (WBCs). Agouti-related protein (AgRP) is an orexigenic peptide secreted predominantly from the arcuate nucleus in the hypothalamus. AgRP affects feeding behavior and plays a role in energy and glucose homeostasis and adiposity. The purpose of the study was to determine effects of circuit resistance exercise (CRE) (9 exercises, 25 s per exercise) at different intensities on peripheral blood lymphocyte (PBL) AgRP mRNA expression and its concentrations in lymphocytes and plasma. Twenty-five young female college students were randomly divided into five groups: control, 40% 1-repetition maximum (1-RM), 60% 1-RM, 80% 1-RM and combined (40 + 60 + 80% 1-RM) loads. Peripheral blood mononuclear cells were isolated by a lymphocyte density gradient centrifugation method for AgRP mRNA expression. Lymphocyte ATP, glycogen, AgRP, growth hormone (GH), and plasma AgRP, GH and glucose concentrations were measured. CRE increased AgRP mRNA lymphocyte expression significantly (P < 0.0001) at all intensities. A higher and significant (P < 0.01) increase was found in the 60% 1-RM group when compared with the other groups. The CRE-induced lymphocyte AgRP expression was accompanied by elevations in plasma AgRP, glucose and GH levels as well as higher WBCs, lymphocytes and neutrophil counts. Lymphocyte AgRP and GH concentrations were significantly reduced (P < 0.05). Lymphocyte ATP content was unchanged and glycogen was reduced in the combined group but not in the other groups. Data indicate that AgRP mRNA is expressed in PBLs and that CRE increases its expression. Data also reveal that the expression of AgRP was accompanied with higher plasma AgRP and GH concentrations. Findings suggest that AgRP may provide an important signal in the immune environment and that the lymphocyte may be considered as an extra-hypothalamic source of plasma AgRP following exercise stress. |
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Authors:
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Abbass Ghanbari-Niaki; Marziyeh Saghebjoo; Amir Rashid-Lamir; Rozita Fathi; Robert R Kraemer |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental biology and medicine (Maywood, N.J.) Volume: 235 ISSN: 1535-3699 ISO Abbreviation: Exp. Biol. Med. (Maywood) Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-04-20 Completed Date: 2010-05-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100973463 Medline TA: Exp Biol Med (Maywood) Country: England |
Other Details:
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Languages: eng Pagination: 326-34 Citation Subset: IM |
Affiliation:
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University of Mazandaran, Babolsar, Mazandaran. ghanbara@yahoo.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Agouti-Related Protein / biosynthesis* Body Mass Index Exercise* Female Gene Expression Regulation* Glucose / metabolism Glycogen / metabolism Growth Hormone / metabolism Humans Leukocytes / metabolism Leukocytes, Mononuclear / cytology Lymphocytes / metabolism* Neutrophils / metabolism Random Allocation |
| Chemical | |
Reg. No./Substance:
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0/Agouti-Related Protein; 50-99-7/Glucose; 9002-72-6/Growth Hormone; 9005-79-2/Glycogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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